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Diazocyclopentadiene (DACP), a light sensitive reagent for the ethylene receptor in plants

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Abstract

Diazocyclopentadiene (DACP) has been shown to be an effective reagent for the ethylene receptor. Treatment of mung bean sprouts or tobacco leaves with DACP in the light or in the dark inactivates much of the ethylene binding. In the light, inactivation seems to be permanent, while in the dark, the site becomes active again after the DACP diffuses away. The compound is 10 times more effective in the light than in the dark. DACP inhibits banana ripening indicating the physiological receptor is involved. It also overcomes the inhibitory effect of ethylene on mung bean seedling growth (Km = 0.09 µl/1 E) at low ethylene levels. At high ethylene levels, an apparent high ethylene level site becomes apparent (Km = 50 µl/1 E) and growth is inhibited.

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References

  1. Burg SP and Burg EA (1967) Molecular requirements for biological activity. Plant Physiol 42: 144–152

    Google Scholar 

  2. Chapman OL and Hess TC (1984) Cyclopentadiene-O-oxide: Spectroscopic observation and photochemistry of a carbonyl oxide. Jour Amer Chem Soc 106: 1842–1843

    Google Scholar 

  3. Doering W von E and DePuy CH (1953) Diazocyclopentadiene. Jour Amer Chem Soc 75: 5955–5957

    Google Scholar 

  4. McElwee-White L and Dougherty DA (1984) Concerning the viability of 1,4,6,9-Spiro [4.4] nonatetrayl as a reactive intermediate: new biradical-to-biradical rearrangements. Jour Amer Chem Soc 106: 3466–3474

    Google Scholar 

  5. Ramirez F and Levy S (1958) Reaction of diazocyclopentadiene with triphenylphosphine. Jour Org Chem 23: 2036–2037

    Google Scholar 

  6. Regitz M and Liedhegener A (1967) Reaktionen aktiver methylenverbindungen mit aziden-XV Synthese von diazocyclopentadienen durch diazogruppenübertagung und einige reaktionen. Tetrahedron 23: 2701–2708

    Google Scholar 

  7. Sanders IO, Smith AR and Hall MA (1989) Ethylene metabolism in Pisum sativum L. Planta 179: 104–114

    Google Scholar 

  8. Sisler EC (1979) Measurement of ethylene binding in plant tissue. Plant Physiol 64: 538–542

    Google Scholar 

  9. Sisler EC (1990) Ethylene binding receptors—is there more than one? In: RP Pharis and SB Rood, eds. Plant Growth Substances, pp. 192–200. Berlin: Springer-Verlag

    Google Scholar 

  10. Sisler EC (1991) Ethylene-binding components in plants. In: AK Matoo and JC Suttle, eds. The Plant Hormone Ethylene, 81–99. Boca Raton: CRC Press

    Google Scholar 

  11. Sisler EC and Blankenship SM (1991a) Action of a photoaffinity label on ethylene processes in plants. Plant Physiol 96S: 158

    Google Scholar 

  12. Sisler EC and Blankenship SM (1991b) Retardation of tomato ripening by a photoaffinity label. Plant Physiol 96S: 158

    Google Scholar 

  13. Sisler EC, Blankenship SM and Guest M (1990) Competition of cyclooctenes and cyclooctadienes for ethylene binding and activity in plants. Plant Growth Reg 9: 157–164

    Google Scholar 

  14. Sisler EC and Wood C (1988) Competition of unsaturated compounds and ethylene for binding and action. Plant Growth Reg 7: 181–191

    Google Scholar 

  15. Sisler EC and Yang SF (1984) Anti-ethylene effects of cis-2-butene and cyclic olefins. Phytochem. 23: 2765–2768

    Google Scholar 

  16. Venis MA (1985) Hormone binding sites in plants. New York: Longman, 191

    Google Scholar 

  17. Warner HL and Leopold AC (1971) Timing of growth responses in peas. Biochim Biophys Res Comm 44: 989–994

    Google Scholar 

  18. Weil T and Cais M (1963) A simplified procedure for the preparation of diazocyclopentadiene and some related compounds. Jour Org Chem 28: 24–72

    Google Scholar 

  19. Young RE, Pratt HK and Biale JB (1952) Manometric determination of low concentrations of ethylene. Anal Chem 24: 511–555

    Google Scholar 

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Sisler, E.C., Blankenship, S.M. Diazocyclopentadiene (DACP), a light sensitive reagent for the ethylene receptor in plants. Plant Growth Regul 12, 125–132 (1993). https://doi.org/10.1007/BF00144593

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  • DOI: https://doi.org/10.1007/BF00144593

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