Abstract
The effect of γ-ketotriazole, MCPA and carbaryl on the lipid composition and ATPase activity associated with plasma membrane fractions from rice (Oryza sativa cv Bahia) shoots was investigated. With γ-ketotriazole and MCPA treatments the relative amount of δ5-avenasterol (%) was reduced in the plasma membrane, whereas with γ-ketotriazole a reduction was also found in the sitosterol content, expressed as a percentage of the total free sterol composition. The fatty acyl chain length of phosphatidylcholine fractions from MCPA-treated plants was also reduced. The plasma membrane Mg2+-ATPase activity was only stimulated in MCPA-treated plants. No changes were observed in lipid composition or ATPase activity with carbaryl treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bradford MH (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analyt Biochem 72: 245–254
Burden RS, Cooke DT, White PJ and James CS (1987) Effects of the growth retardant tetcyclacis on the sterol composition of oat (Avena sativa). Plant Growth Reg 5: 207–217
Cooke DT, Burden RS, Clarkson DT and James CS (1989) Xenobiotic-induced changes in membrane lipid composition: effect on plasma membrane ATPases. In: RK Atkin and DR Clifford, eds. Mechanism and Regulation of Transport Processes. BPGR Group Mono 18: 41–53
Cooke DT and Burden RS (1990) Lipid modulation of plasma membrane-bound ATPases. Physiol Plant 78: 153–159
Cooke DT, Munkonge FM, Burden RS and James CS (1991) Fluidity and lipid composition of oat and rye shoot plasma membranes: Effect of lipid perturbation by xenobiotics. Biochim Biophys Acta 106: 156–162
Larsson C, Widell S and Kjellbom P (1987) Preparation of high-purity plasma membranes. Methods Enzymol 148: 558–568
Ros R, Cooke DT, Burden RS and James CS (1990) Effect of the herbicide MCPA, and the heavy metals cadmium and nickel on the lipid composition, Mg2+-ATPase activity and fluidity of plasma membrane from rice, Oryza saliva (cf. Bahia) shoots. J Exp Bot 41: 457–462
Sze H (1985) H+ translocating ATPase: advances using membrane vesicles. Annu Rev Plant Physiol 36: 175–208
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ros, R., Cooke, D.T., Burden, R.S. et al. A comparison of the effects of γ-ketotriazole, MCPA and carbaryl on the plasma membrane Mg2+-ATPase activity and lipid composition of rice shoots (Oryza sativa). Plant Growth Regul 12, 67–69 (1993). https://doi.org/10.1007/BF00144584
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00144584