Abstract
Methods are described for obtaining explants which produce adventitious shoots, for subsequent stimulation of rooting and then transplanting using six commercial sugar-beet cultivars. The rate of adventitious shoot regeneration from petioles or intact leaf explants was affected by the source of donor plants, cytokinin type (BAP or Kin) and concentration and cultivar. Increasing the sucrose concentration of the medium from 3% to 5% or 8% had no apparent effect. Adventitious shoots could be produced directly from callus formed on the base of the petioles. In general adventitious shoots were produced on either the concave surface of the petiole or from the callus, occasionally simultaneously on both, and on the convex surface of the petiole in intact leaf explants. The highest rooting rate with 3% sucrose and 1.0 mg l−1 NAA was obtained using half-strength MS medium. There was considerable variation in the propagules from petioles or callus indicating that this system may provide valuable somaclonal variation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BAP:
-
benzylaminopurine
- IBA:
-
indole-3-butyric acid
- GA3 :
-
gibberellic acid
- MS:
-
Murashige and Skoog medium
- NAA:
-
naphthaleneacetic acid
References
Coumans-Gilles MF, Kevers C, Coumans M, Ceulemans E and Gaspar T (1981) Vegetative multiplication of sugar beet through in vitro culture of inflorescence pieces. Pl Cell Tiss Org Culture 1, 2: 93–101.
Freytag AH, Anand SC, Rao-Arelli AP and Owens LD (1988) An improved medium for adventitious shoot formation and callus induction in Beta vulgaris L. in vitro. Pl Cell Rep 7: 30–34.
Harms CT, Baktir I and Oertli JJ (1983) Clonal propagation in vitro of red beet (Beta vulgaris) by multiple adventitious shoot formation. Pl Cell Tiss Org Culture 2: 93–102.
Hussey G and Hepher A (1978) Clonal propagation of sugar-beet plants and the formation of polyploids by tissue culture. Ann Bot 42: 477–479.
Miedema P, Groot PJ and Zuidgeest JHM (1980) Vegetative propagation of Beta vulgaris by leaf cuttings. Euphytica 29:425–432.
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–495.
Ritchie GA, Short KC and Davey MR (1989) In vitro shoot regeneration from callus leaf axils and petioles of sugar beet (Beta vulgaris L.). J Expt Bot 40, No. 211: 277–283.
Saunders JW (1982) A flexible in vitro shoot culture propatation system for sugar beet that includes rapid floral induction of ramets. Crop Sci 22: 1102–1105.
Saunders JW and Doley WP (1986) One step shoot regeneration from callus of whole plant leaf explants of sugar beet lines and a somoclonal variant for in vitro behaviour. J Plant Physiol 124: 473–479.
Saunders JW and Shin K (1986) Germplasm and physiological effects on induction of high-frequency hormone autonomous callus and subsequent shoot regeneration in sugar beet. Crop Sci 26: 1240–1245.
Slavova IV, Zahariev A and Antonova G (1982) Improving and applying the method of sugar beet vegetative propagation in vitro. Plant Physiol BAS (Sofia) 7: 95–99.
Tetu T, Sangwan RS and Sangwan-Norreel BS (1987) Hormonal control of organogenes and somatic embryogenesis in Beta vulgaris callus. J Expt Bot 38: No. 188, 506–5xx.
Xie Qi Kun (1986) Tissue culture of medical plants. Shanghai Sci Technol Pub House: p 32.
Yu MH (1989) Callus induction and differentiation from leaf explants of different species of the genus Beta. Crop Sci 29: 205–209.
Author information
Authors and Affiliations
Additional information
Author for correspondence
Rights and permissions
About this article
Cite this article
Zhong, Z., Smith, H.G. & Thomas, T.H. In vitro culture of petioles and intact leaves of sugar beet (Beta vulgaris). Plant Growth Regul 12, 59–66 (1993). https://doi.org/10.1007/BF00144583
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00144583