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Effects of kinetin, paclobutrazol and their interactions on the microtuberization of potato stem segments cultured in vitro in the light

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Abstract

Single-nodal cuttings of Solanum tuberosum (four cultivars) and Solanum chacoense were induced to produce in vitro microtubers on Murashige & Skoog (MS) medium supplemented with 8 g l−1 sucrose and various concentrations of kinetin and paclobutrazol. The cultures were kept 10 days in darkness and then transferred to a 14 h daylength with 100 µE m−2 sec−1 light intensity at 21 °C. Kinetin (2.5 mg l−1) had no significant influence on tuber formation. However, its addition together with paclobutrazol (0.001 mg l−1) significantly enhanced tuberization. Paclobutrazol alone stimulated early tuber initiation and inhibited stem growth. Despite some genotype × treatment interactions, all genotypes (from very early to late and wild type) formed the maximum proportion of explants bearing microtubers on the media containing both plant growth regulators.

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  1. Department of Genetics & Plant Breeding, University of Agriculture, Lomonosovova 2, 949 76, Nitra, Czechoslovakia

    I. Šimko

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  1. I. Šimko
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Šimko, I. Effects of kinetin, paclobutrazol and their interactions on the microtuberization of potato stem segments cultured in vitro in the light. Plant Growth Regul 12, 23–27 (1993). https://doi.org/10.1007/BF00144578

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  • DOI: https://doi.org/10.1007/BF00144578

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