Summary
Germinated seeds of Onopordum turcicum, which is a kind of thistle, were induced to develop callus on Murashige-Skoog medium. Both callus and suspension cultures of the plant synthesized extracellular and intracellular protease as a product of primary metabolism. The proteolytic activities of the enzyme complex from cell suspension and callus cultures were higher than those from the leaves and seeds of the plant.
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Tamer, I.M., Mavituna, F. Protease from callus and cell suspension cultures of Onopordum turcicum (Compositae). Biotechnol Lett 18, 361–366 (1996). https://doi.org/10.1007/BF00143452
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DOI: https://doi.org/10.1007/BF00143452