Abstract
Research on Xanthomonas oryzae pv. oryzae, the bacterial blight of rice pathogen, was initiated at the Institute of Agriculture and Animal Science (IAAS) with the main objective of assessing the population structure of X. o. pv. oryzae through the use of both conventional and molecular markers in combination with virulence typing. A high DNA polymorphism was detected in the pathogen populations using different DNA probes and rep-PCR primers. Most strains were avirulent to cultivars containing the bacterial blight resistance gene Xa-21, which suggested the strategy that targets gene deployment is feasible in Nepal.
Similar content being viewed by others
References
AdhikariT.B. (1986) Reaction of rice cultivars to bacterial blight. Int. Rice Res. Inst. Newslett. 11, 15–16.
AdhikariT.B. (1993) Assessment of near-isogenic rice lines for resistance to Xanthomonas oryzae pv. oryzae in Nepal. Int. J. Pest Manage. 39, 293–6.
Adhikari, T.B. and Mew, T.W. (1988) Research on bacterial blight of rice in Nepal. In Proc. Int. Cong. Plant Pathol. 5th, Kyoto, Japan. p. 98.
AdhikariT.B. and MewT.W. (1991) Effect of bacterial blight on growth and yield of rice. J. Inst. Agricult. Animal Sci. 12, 29–40.
AdhikariT.B. and MewT.W. (1994) Resistance of rice to Xanthomonas oryzae pv. oryzae in Nepal. Plant Dis. 78, 68–7.
AdhikariT.B. and ShresthaS.M. (1989) Distribution and severity of bacterial blight of rice in Nepal. J. Inst. Agricult. Animal Sci. 10, 31–8.
AdhikariT.B., MewT.W. and TengP.S. (1994a) Phenotypic diversity of Xanthomonas oryzae pv. oryzae in Nepal. Plant Dis. 78, 68–72.
AdhikariT.B., MewT.W. and TengP.S. (1994b) Progress of bacterial blight on rice cultivars carrying different Xa genes for resistance in the field. Plant Dis. 78, 73–7.
AdhikariT.B., Vera CruzC.M., ZhangQ., NelsonR.J., SkinnerD.Z., MewT.W. and LeachJ.E. (1995) Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia. Appl. Environ. Microbiol. 61, 966–71.
ACRIP (All India Coordinated Rice Improvement Project) (1971) Progress Report for 1971. Hyderabad, India: ACRIP.
ChandT., SinghN., SinghH. and ThindB.S. (1979), Field efficacy of stable bleaching powder to control bacterial blight of rice. Int. Rice. Res. Inst. Newslett. 4, 12.
deBruijnF.J. (1992) Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti and other soil bacteria, Appl. Environ. Microbiol. 58, 2180–7.
ExcondeO.R., OpinaO.S. and PhanomsawaraA. (1971) Yield losses due to bacterial leaf blight, Philipp. Agricult. 57, 120–40.
IRRI (International Rice Research Institute) (1979) Rice Disease in North and Eastern India, Nepal, and Bangladesh with Focus on Bacterial Blight. Los Banos, Philippines: International Rice Research Institute.
KhadkaB.B., ShahS.M. and LawatK. (1968) Plant Diseases in Nepal — a Supplementary List. Plant Protection Bulletin Technical Document, No. 66, Rome: FAO.
LeachJ.E., RhoadsM.L., Vera CruzC.M., WhiteF.F., MewT.W. and LeungH. (1992) Assessment of genetic diversity and population structure of Xanthomonas oryzae pv. oryzae with a repetitive DNA element. Appl. Environ. Microbiol. 58, 2188–95.
LeachJ.E., NelsonR.J., LeungH. and MewT.W. (1995) Population biology of Xanthomonas oryzae pv. oryzae and approaches to its control. Curr. Opin. Biotechnol. 6, 298–304.
LouwsF.J., FulbrightD.W., StephensC. and deBruijnF.J. (1994) Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl. Environ. Microbiol. 60, 2286–95.
ManandharH.K., ThapaB.J. and AmatyaP. (1987) Bacterial blight in hilly regions of Nepal. Int. Rice Res. Inst. Newslett. 12, 28.
MewT.W. (1987) Current status and future prospects of research on bacterial blight of rice. Annu. Rev. Phytopathol. 25, 359–82.
MewT.W., AlvarezA.M., LeachJ.E. and SwingsJ. (1993) Focus on bacterial blight of rice. Plant Dis. 77, 5–12.
MizukamiT. and WakimotoS. (1969) Epidemiology and control of bacterial leaf blight of rice, Annu. Rev. Phytopathol. 7, 51–72.
MundtC.C. (1994) Use of genetic diversity to control cereal diseases: implication on rice blast. In R.S.Zeigler, S.A.Leong and P.S.Teng (eds) Rice blast disease, UK, CAB International.
NelsonR.J., BaraoidanM.R., Vera CruzC.M., YapI.P., LeachJ.E., MewT.W. and LeungH. (1994) Relationship between phylogeny and pathotype for bacterial blight pathogen of rice. Appl. Environ. Microbiol. 60, 3275–83.
OuS.H. (1982) Rice Diseases, 2nd edn. Kew, Surrey: Commonwealth Mycological Institute.
ReddyR. and YinS.-Z. (1989) Survival of Xanthomonas campestris pv. oryzae, the causal organism of bacterial leaf blight of rice. In Bacterial blight of rice, pp. 65–78. Los Banos, Philippines: IRRI.
ReddyA.P.K., MackenzieD.R., RouseD.R. and RaoA.V. (1979) Relationship of bacterial blight severity to grain yield of rice. Phytopathology 69, 967–9.
RehmanF.U., BenedictA.A. and AlvarezA.M. (1992) New serogroups of Xanthomonas oryzae pv. oryzae detected with monoclonal antibodies. Phytopathology 82, 1144.
RohlfF.J. (1992) NTSYS-PC. Numerical Taxonomy and Multivariate Analysis System. Version 1.70 Setauket, NY: Exeter Software.
SAS Institute, Inc. (1989) SAT/TAT User's Guide, 4th edn, Vol. 1. Cary, NC: SAS Institute, Inc.
SwingsJ., Van DenMooterM., VauterinL., HosteB., GillisM., MewT.W. and KerstersK. (1990) Reclassification of the causal agents of bacterial blight (Xanthomonas campestris pv. oryzae) and bacterial leaf streak (Xanthomonas campestris pv. oryzicola) of rice as pathovars of Xanthomonas oryzae (ex Ishiyama, 1922) sp. nov. nom. rev. Int. J. Syst. Bacteriol. 40, 309–11.
Vera Cruz, C.M., Halda, L., Louws, F., Skinner, D.Z., George, M.L., Nelson, R.J., de Bruijn, F.J., Rice, C. and Leach, J.E. (1995) Repetitive sequence-based PCR of Xanthomonas monas oryzae pv. oryzae and Pseudomonas species. Int. Rice Res. Inst. Note. in press.
VersalovicJ., SchneiderM., deBruijnF. and LupskiJ.R. (1994) Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol. Cell. Biol. 5, 25–40.
WolfeM.S. (1985) The current status and prospects of multiline cultivars and variety mixtures for disease resistance. Annu. Rev. Phytopathol. 25, 251–73.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Adhikari, T., Leach, J. & Mew, T.W. Bacterial blight of rice and its control in Nepal. Integr Pest Manag Rev 1, 91–95 (1996). https://doi.org/10.1007/BF00142827
Issue Date:
DOI: https://doi.org/10.1007/BF00142827