Research on Xanthomonas oryzae pv. oryzae, the bacterial blight of rice pathogen, was initiated at the Institute of Agriculture and Animal Science (IAAS) with the main objective of assessing the population structure of X. o. pv. oryzae through the use of both conventional and molecular markers in combination with virulence typing. A high DNA polymorphism was detected in the pathogen populations using different DNA probes and rep-PCR primers. Most strains were avirulent to cultivars containing the bacterial blight resistance gene Xa-21, which suggested the strategy that targets gene deployment is feasible in Nepal.
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Adhikari, T., Leach, J. & Mew, T.W. Bacterial blight of rice and its control in Nepal. Integr Pest Manag Rev 1, 91–95 (1996). https://doi.org/10.1007/BF00142827
- disease resistance
- genetic diversity
- RFLP analysis
- Xanthomonas oryzae pv. oryzae