Abstract
Activation of bovine peripheral blood mononuclear cells (PBM) towards immunoglobulin (Ig) synthesis and secretion was examined in vitro using pokeweed mitogen (PWM) or PWM plus sheep red blood cells (SRBC). Bovine PBM were composed of 7–30% B cells, 25–56% T cells and 1–4% macrophages. Cell density and PWM concentrations were critical parameters for obtaining reproducible maximum lymphocyte proliferation and polyclonal B cell activation. Inclusion of aminopterin, a folic acid antagonist, reduced cellular proliferation and viability but had no apparent effect on the time of appearance of peak proliferation. Co-culture of PBM with SRBC and PWM generated anti-SRBC specific antibodies.
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References
Bielefeldt Ohmann, H., Filion, L.G. and Babiuk, L.A., 1983. Bovine monocytes and macrophages: An accessory role in suppressor cell generation by Con A and in lectin-induced proliferation. Immunology, 50, 189–197
Bonner, W.A., Hulett, H.R., Sweet, R.G. and Herzenberg, L.A., 1972. Fluorescence activated cell sorting. Review of Scientific Instruments, 43, 404–409
Coutinho, A. and Moller, G., 1973. B-cell mitogenic properties of thymus-independent antigen. Nature, 245, 12–21
Cunningham, A.J. and Szenberg, A., 1968. Further improvement in the plaque technique for detecting single antibody forming cells. Immunology, 14, 599–600
Evans, H.J. and Vijayalaxmi, 1981. Induction of 8-azaguanine resistance and sister chromatid exchange in human lymphocytes exposed to mitomycin C and X-rays in vitro. Nature, 292, 601–605
Grewal, A.S., Rouse, B.T. and Babiuk, L.A., 1976. Erythrocyte rosettes — A marker for bovine T cells. Canadian Journal of Comparative Medicine, 40, 298–305
Lalor, P., 1983. Monoclonal antibodies to bovine leucocyte membrane antigens define heterogeneity of target cells for Theileria parva parasitosis, (PhD thesis, Brunel University)
Lipsky, P.E., 1980. Staphylococcal protein A, a T cell-regulated polyclonal activator of human B cells. Journal of Immunology, 125, 155–162
Muscoplat, C.C., Alhaji, I., Johnson, D.W., Pomeroy, K.A., Olsson, J.M., Larson, V.L., Stevens, J.D. and Sorensen, D.K., 1974. Characteristics of lymphocyte responses to phytomitogens: Comparison of responses of lymphocytes from normal and lymphocytic cows. American Journal of Veterinary Research, 35, 1053–1055
Olsson, L., Kronstrom, H., Mouzon, C., Honsik, C., Brodin, T. and Jakobson, B., 1983. Antibody producing human-human hybridomas. 1. Technical aspects. Journal of Immunological Methods, 61, 17–32
Outteridge, P.M. and Dufy, J.H., 1981. Surface markers for characterisation of bovine blood lymphocyte populations and change in this from birth to maturity. Research in Veterinary Science, 31, 315–322
Pearson, T.W., Roelants, G.E., Lundin, L.B. and Mayor-Withey, K.S., 1979. The bovine lymphoid system: Binding and stimulation of peripheral blood lymphocytes by lectins. Journal of Immunological Methods, 26, 271–282
Pinder, M., Musoke, A.J., Morrison, W.I. and Roelants, G.E., 1980. The bovine lymphoid system. III. A monoclonal antibody specific for bovine cell surface and serum IgM. Immunology, 40, 359–365
Schalm, O.W., Jain, N.C. and Carrol, E.J., 1975. Veterinary Haematology, (Lea and Febiger, Philadelphia), 82–216
Waldmann, T.A. and Broder, S., 1982. Polyclonal B-cell activators in the study of the regulation of immunoglobulin synthesis in the human system. Advances in Immunology, 32, 1–55
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Olobo, J.O., Black, S.J. In vitro secretion of bovine immunoglobulins during pokeweed mitogen or pokeweed mitogen and antigen activation of lymphocytes. Vet Res Commun 13, 193–197 (1989). https://doi.org/10.1007/BF00142044
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DOI: https://doi.org/10.1007/BF00142044
Keywords
- antigen
- cattle
- immunity
- immunoglobulin
- lymphocytes
- mitogen