Skip to main content
Log in

Effects of initial maltose concentration, agitation and aeration rates on the production of glucosyltransferase byAspergillus niger CCRC 31494

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

The microorganism Aspergillus niger CCRC 31494 can produce an extracellular glucosyltransferase (GTase, EC 2.4.1.24) with a high transglucosylating activity. The maximal enzyme production occurred at initial maltose concentration of 40 gl-1. The microorganism was also grown in a 5-liter jar fermenter for GTase production. It was found that the optimal agitation and aeration rates were 750 rev min-1 and 1.0 l min-1, respectively, and the enzyme production was about 0.25–0.26 units ml-1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Banerjee, U. C. (1993) Biotechnol. Techniques, 10, 733–738

    Article  Google Scholar 

  • Benson, C. P., Kelly, T., and Fogarty, W. M. (1982) J. Chem. Technol. Biotechnol., 32, 790–798

    CAS  Google Scholar 

  • Chen, W. C. and Hung, T. F. (1995) The 33rd annual meeting of Chin. Agric. Chem. Soc., A-4, p. 84, Taipei, Taiwan.

  • Chen, W. C., Liu, C. H., and Huang, C. N. (1994) J. Chin. Agric. Chem. Soc., 32, 639–646

    CAS  Google Scholar 

  • Chiba, S. and Shimomura, T. (1979) Denpun Kagaku, 26, 59–67

    CAS  Google Scholar 

  • Hang, Y. D. and Woodams, D. D. (1995) Biotechnol. Lett., 17, 1335–1336

    CAS  Google Scholar 

  • Hayashi, S., Hinotani, T., Hayashi, T., Takasaki Y., and Imada, K. (1993) World J. Microbiol. Biotechnol., 9, 248–250

    Article  CAS  Google Scholar 

  • Hideo, T. (1994) Food Technol., 48, 61–65

    Google Scholar 

  • Kanaya, K., Chiba, S., and Shimomura, T. (1976) Agric. Biol. Chem., 40, 1929–1936

    CAS  Google Scholar 

  • Kitahata, S., Murakami, H., and Okada, S. (1989) ibid., Agric. Biol. Chem., 53, 2653–2659.

  • Kuriki, T., Tsuda, M., and Imanaka, T. (1992) J. Ferment. Bioeng., 73, 198–202

    Article  CAS  Google Scholar 

  • Kuriki, T., Yanase, M., Takata, H., Takesada, Y., Imanaka, T., and Okada, S. (1993) Appl. Environ. Microbiol., 59, 953–959

    PubMed  CAS  Google Scholar 

  • McCleary, V., Gibson, S., Sheehan, H., Casey, A., Horgan, L., and O'flaherty, J. (1989) Carbohydr. Res., 185, 147–162

    Article  CAS  Google Scholar 

  • Miller, G. L. (1959) Anal. Chem., 31, 426–427

    Article  CAS  Google Scholar 

  • Pazur, J. H. and French, D. (1951) J. Am. Chem. Soc., 73, 3536

    Article  CAS  Google Scholar 

  • Pazur, J. H. and Okada, S. (1967) Carbohydr. Res., 4, 371–379

    Article  CAS  Google Scholar 

  • Pazur, J. H. and Okada, S. (1968) J. Biol. Chem., 243, 4732–4738

    PubMed  CAS  Google Scholar 

  • Pazur, J. H., Tominaga, Y., Debrosse, C. W., and Jackman, L. M. (1978) Carbohydr. Res., 61, 279–290

    Article  CAS  Google Scholar 

  • Stetten, M. R. (1959) J. Am. Chem. Soc., 81, 1437–1441

    Article  CAS  Google Scholar 

  • Vamos, L. and Rose, P. (1973) Starke, 25, 195–202

    Article  CAS  Google Scholar 

  • Yan, T. R. and Liu, D. Z. (1993) J. Chin. Biochem. Soc., 22, 55–67

    CAS  Google Scholar 

  • Yun, J. W., Lee, M. G., and Dong, S. K. (1994a) Biotechnol. Lett., 16, 359–362

    CAS  Google Scholar 

  • Yun, J. W., Lee, M. G, and Dong, S. K. (1994b) ibid. Biotechnol. Lett., 16, 1145–1150

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, WC., Lee, SL. & Tung, PY. Effects of initial maltose concentration, agitation and aeration rates on the production of glucosyltransferase byAspergillus niger CCRC 31494. Biotechnol Lett 18, 489–494 (1996). https://doi.org/10.1007/BF00140189

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00140189

Keywords