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Affinity chromatography of extracellular cellulase from chaetomium cellulolyticum

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Summary

An endo-β-glucanase of C.cellulolyticum was purified by a procedure involving concanavalin A (Con A)-Sepharose chromatography and polyacrylamide gel electrophoresis (PAGE). The enzyme produced G1 and oligosaccharides from CMC. Chromatography on Procion Red HE3B-Agarose proved to be useful in the separation of cellobiase from cellobiose dehydrogenase.

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Abbreviations

CMC:

carboxymethyl cellulose

CM-cellulase:

carboxymethyl cellulase

FP-cellulase:

filter paper degrading cellulase

G1:

glucose

G2:

cellobiose

G3:

cellotriose

G4:

cellotetraose

G5:

cellopentaose

G6:

cellohexaose

G7:

celloheptaose

α-MG:

methyl-α-D-glucoside

pNPG:

p-nitrophenyl-β-glucopyranoside

pNP:

p-nitrophenol

CBDH:

cellobiose dehydrogenase

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Authors and Affiliations

  1. Institute of Biotechnology, Nuclear Research Centre Jülich, P.O. Box 1913, D-5170, Jülich, Federal Republic Germany

    P. Fähnrich & K. Irrgang

Authors
  1. P. Fähnrich
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  2. K. Irrgang
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Fähnrich, P., Irrgang, K. Affinity chromatography of extracellular cellulase from chaetomium cellulolyticum. Biotechnol Lett 6, 251–256 (1984). https://doi.org/10.1007/BF00140046

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