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A novel α-glucosidase produced by Bacillus amylolyticus

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Abstract

Bacillus amylolyticus produces α-amylase, pullulanase and α-glucosidase. By selection of carbon source in the growth medium, α-glucosidase was produced preferentially and with exclusion of the other two activities. The α-glucosidase was highly specific for maltose and to a lesser extent maltotriose but was inactive towards a range of other substrates including p-nitrophenyl α-D-glucoside and isomaltose. Optima for activity were recorded at pH 7.0 and 40° C and the enzyme was insensitive to ethylenediaminetetraacetic acid.

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Authors and Affiliations

  1. Department of Industrial Microbiology, University College, 4, Belfield, Dublin, Ireland

    C. T. Kelly, M. E. Heffernan & W. M. Fogarty

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  1. C. T. Kelly
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  2. M. E. Heffernan
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  3. W. M. Fogarty
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Kelly, C.T., Heffernan, M.E. & Fogarty, W.M. A novel α-glucosidase produced by Bacillus amylolyticus . Biotechnol Lett 2, 351–356 (1980). https://doi.org/10.1007/BF00138668

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