Summary
The possibility that adenosine 3′,5′ monophosphate exerts an effect on citric acid production by Aspergillus niger by influencing pellet morphology has been investigated. The effect of pH and inoculum size on pellet formation, citric acid production, and intracellular and extracellular cAMP levels were studied. High levels of intracellular and extracellular cAMP in the later stages of the fermentation, the period of maximum citric acid formation, were associated with those treatments which gave pellets of intermediate size. The highest cAMP levels were associated with those treatments which gave the highest citric acid titre. It was concluded that high cAMP levels are principally associated with an optimum physiological state for citric acid production and that cAMP levels do not vary directly with pellet size.
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Al Obaidi, Z.S. (1977). Ph.D. Thesis, University of Strathclyde.
Berry, D.R., Chmiel, A. and Al Obaidi, Z. S. (1977). In: Genetics and Physiology of Aspergillus. Ed. J.E. Smith and J.A. Pateman 405–426 Academic Press.
Gilman, A.G. (1970). Proc. Nat. Acad. Sci., U.S.A. 67, 305–312
Larsen, A.D. and Sypherd, P.S. (1974). J. Bacteriol. 117, 432–438
Mansour, T.E. (1969). Advances in Enzyme Regulation. 8, 37–51.
Schlanderer, G. and Dellweg, H. (1974). Europ. J. of Biochem. 49, 365–316.
Shu, P. and Johnson, M.J. (1947). J. Bacteriol. 54, 161–167.
Shu, P. and Johnson, M.J. (1948). J. Bacteriol. 56, 577–585
Tellez-Inon, M.T. and Torres, H.M. (1970). Proc. Nat. Acad. Sci., U.S.A. 66, 459–463.
Torres, H.N. Flawia, M.M., Terenzi, H.P. and Tellez-Inon, M.T. (1975). In: Cyclic Nucleotide Research. Ed. G.I. Drummond, P. Greengard and G.A. Robinson. 5, 67–78. Raven Press.
Van der Plaat, J.B. and Van Solingen, P. (1974). Biochem. Biophys. Res. Commun. 56, 580–586.
Wayne, P.D. and Rosen, O.M. (1974). Proc. Nat. Acad. Sci. U.S.A. 71, 1436–1440.
Wold, W.S.M. and Suzuki, I. (1973a). Biochem. Biophys. Res. Commun. 50, 237–244.
Wold, W.S.M. and Suzuki, I. (1973b). Biochem. and Biophys. Res. Commun. 55, 824–830.
Wold, W.S.M. and Suzuki, I. (1976). Canad. J. of Microbiol. 22, 1093–1101.
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Al Obaidi, Z.S., Berry, D.R. cAMP concentration, morphological differentiation and citric acid production in Aspergillus niger . Biotechnol Lett 2, 5–10 (1980). https://doi.org/10.1007/BF00136876
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DOI: https://doi.org/10.1007/BF00136876