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Effects of culture conditions on molecular weights of poly(3-hydroxyalkanoates) produced byPseudomonas putida from octanoate

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Summary

Time-dependent changes in the molecular weights of poly(3-hydroxyalkanoates) (P(3HA)) produced byPseudomonas putida from octanoate were investigated. The mole ratio of carbon to nitrogen sources (C/N), incubation temperature, concentration of octanoate, and pH of culture solution were varied to study the effects of culture conditions on the yield and molecular weights of P(3HA). The molecular weights of P(3HA) decreased with time to reach a constant value during incubation. When the incubation temperature and the concentration of octanoate were low, P(3HA) polymers of high molecular weights were produced.

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References

  • Brandl, H., Gross, R. A., Lenz, R. W. and Fuller, R. C. (1988) Appl. Environ. Microbiol. 54, 1977–1982

    PubMed  CAS  Google Scholar 

  • Braunegg, G., Sonnleitner, B. and Lafferty, R. M. (1978) Eur. J. Appl. Microbiol. 6, 29–37

    Article  CAS  Google Scholar 

  • de Smet, M. J., Eggink, G., Witholt, B., Kingma, J. and Wynberg, H. (1983) J. Bacteriol. 154, 870–878

    PubMed  Google Scholar 

  • de Waard, P., van der Wal., H., Huijberts, G. N. M. and Eggink, G. (1993) J. Biol. Chem. 268, 315–319

    PubMed  Google Scholar 

  • Haywood, G. W., Anderson, A. J. and Dawes, E. A. (1989) Biotechnol. Lett. 11, 471–476

    Article  CAS  Google Scholar 

  • Haywood, G. W., Anderson, A. J., Ewing, D. F. and Dawes, E. A. (1990) Appl. Environ. Microbiol. 56, 3354–3359

    PubMed  CAS  Google Scholar 

  • Huijberts, G. N. M., Eggink, G., de Waard, P., Huisman, G. W. and Withlot, B. (1992) Appl. Environ. Microbiol. 58, 536–544

    PubMed  CAS  Google Scholar 

  • Huisman, G. W., de Leeuw, O., Eggink, G. and Witholt, B. (1989) Appl. Environ. Microbiol. 55, 1949–1954

    PubMed  CAS  Google Scholar 

  • Kawaguchi, Y. and Doi, Y. (1992) Macromolecules 25, 2324–2329

    Article  CAS  Google Scholar 

  • Lageveen, R. G., Huisman, G. W., Preusting, H., Ketelaar, P., Egginnk, G. and Witholt, B. (1988) Appl. Environ. Microbiol. 54, 2924–2932

    PubMed  CAS  Google Scholar 

  • Sonnleiter, B., Heinzle, E., Braunegg, G. and Lafferty, R. M. (1979) Appl. Microbiol. Biotechnol. 7, 1–7

    Article  Google Scholar 

  • Steinbüchel, A. (1991a) Nachrichtenbl. Chem. Tech. Lab. 39, 1112–1114

    Google Scholar 

  • Steinbüchel, A. (1991b) Polyhydroxyalkanoic acids. In; Byrom D (ed) Biomaterials. Macmillan, London, pp 123–213

    Google Scholar 

  • Timm, A. and Steinbüchel, A. (1990) Appl. Environ. Microbiol. 56, 3360–3366

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, 227, Yokohama, Japan

    Katsutoshi Hori & Kazuo Soga

  2. Polymer Chemistry Laboratory, The Institute of Physical Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, 351-01, Saitama, Japan

    Yoshiharu Doi

Authors
  1. Katsutoshi Hori
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  2. Kazuo Soga
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  3. Yoshiharu Doi
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Hori, K., Soga, K. & Doi, Y. Effects of culture conditions on molecular weights of poly(3-hydroxyalkanoates) produced byPseudomonas putida from octanoate. Biotechnol Lett 16, 709–714 (1994). https://doi.org/10.1007/BF00136476

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  • DOI: https://doi.org/10.1007/BF00136476

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