Summary
Fed-batch fermentations were performed to produce hirudin, an anticoagulant protein, from recombinantS. cerevisiae. The structural gene coding for hirudin was combined with theGAL10 promoter for controlled expression and theMFα1 signal sequence for secretion to the growth medium. Control of galactose concentration in a fed-batch mode of operation yielded 110 g/L of final cell density and 260 mg/L of maximum hirudin concentration in the medium, which corresponds to a 3.5-fold increase in cell density and a 4.1-fold enhancement in hirudin concentration compared with the batch fermentation.
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References
Markwardt, F. 1970. Hirudin as an Inhibitor of Thrombin. p.924–932. in G.E. Perlmann and L Lorand (eds.), Methods in Enzymol., vol. 19. Academic Press, New York, London.
Markwardt, F., Nowak, J., Sturzebecher, J., Walsmann, P., and Vogel, G. (1984) Thromb. Haemostasis. 52: 160–170.
Modak, J.M., and Lim, H.C. 1989. Biotchnol. Bioeng. 33: 11–15.
Patkar, A.Y., and Seo, J.H. 1992. Biotechnol. Bioeng. 40: 103–109.
Patkar, A.Y., Shin, P.K., and Seo, J.H. 1990. Kor. J. Appl. Microbiol. Biotech. 18: 394–400.
Sohn, J.H., Lee, S.K., Choi, E.S., and Rhee, S.K. 1991. J. Microbiol. Biotechnol. 1: 266–273.
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Lee, DH., Park, JB., Seo, JH. et al. Expression of hirudin in fed-batch cultures of recombinantSaccharomyces cerevisiae . Biotechnol Lett 16, 667–670 (1994). https://doi.org/10.1007/BF00136468
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DOI: https://doi.org/10.1007/BF00136468