Summary
Improved glucoamylase productivity was screened for using surface cultures in small tubes containing Dextran T-10 broth buffered at a pH supra-optimal for activity of the glucoamylase of the parental strain. Visual identification of improved mutations on microtitre plates is based on their relative abilities to produce residual glucose in culture filtrates. A 141% increase in glucoamylase was achieved.
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Kuek, C., Kidby, D.K. Screening fungi for improved glucoamylase productivity using buffered Dextran broth cultures. Biotechnol Lett 6, 561–566 (1984). https://doi.org/10.1007/BF00135682
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DOI: https://doi.org/10.1007/BF00135682