Abstract
The location of heterochromatin in the chromosomes of Petunia hybrida (2n=14) is presented. C-banded mitotic metaphase chromosomes and carmine-stained pachytene bivalents have been studied. It is shown that the heterochromatin is predominantly located near the centromeres and at the secondary constrictions of the satellite chromosomes. The distribution of chromomeres in pachytene bivalents also reveals that heterochromatin is not restricted to distinct blocks, as is the case in tomato, but occurs in smaller chromomeres which gradually decrease in size towards the ends. Conspicuous telomeres have not been observed. Both C-banding technique and pachytene analysis demonstrate large variation of heterochromatin between different lines of Petunia. The study of pachytene morphology has been hampered by a high degree of non-specific stickiness of the bivalents. Both techniques prove to be unsuitable tools for large-scale chromosome identification of Petunia lines.
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References
Brown, S. W., 1949. The structure and meiotic behavior of the differentiated chromosomes of tomato. Genetics 34: 437–461.
Brown, S. W., 1966. Heterochromatin. Science 151: 417–425.
Engels, F. M. & Croes, A. F., 1968. The SC in yeast. Chromosoma 25: 104–106.
Fussel, C. P., 1977. Telomere associations in interphase nuclei of Allium cepa demonstrated by C-banding. Expl Cell Res. 110: 111–117.
Gottschalk, W., 1954. Die Chromosomenstruktur der Solanaceen unter Berücksichtigung phylogenetischer Fragestellungen. Chromosoma 6: 539–626.
Maizonnier, D., 1971. Utilisation de plantes haploïdes pour l'analyse du caryogramme de Petunia hybrida Hort. Annls Amél. Pl. 21: 257–264.
Marthaler, H., 1937. Morphologie der Chromosomen des Zellkernes von Petunia. Z. indukt. Abstamm.-u. Vererb-Lehre 72: 258–266.
Ramanna, M. S. & Wagenvoort, M., 1976. Identification of the trisomic series in diploid Solanum tuberosum L. Group Tuberosum. I. Chromosome identification. Euphytica 25: 233–240.
Smith, F. J. & Oud, J. L., 1972. The possibility to distinguish chromosomes of Petunia hybrida by quinacrine fluorescence. Genetica 43: 589–596.
Smith, F. J., Oud, J. L. & De, Jong, J. H., 1973. A standard karyogram of Petunia hybrida Hort. Genetica 44: 474–484.
Smith, F. J., De, Jong, J. H. & Oud, J. L., 1975. The use of primary trisomics for the localization of genes on the seven different chromosomes of Petunia hybrida. I. Triplo V. Genetica 45: 361–370.
Snow, R., 1963. Alcoholic hydrochloric acid-carmine as a stain for chromosomes in squash preparations. Stain Technol. 38: 9–13.
Venkateswarlu, J. & Raja Rao, K. G., 1977. Morphology of the pachytene chromosomes of Physalis philadelphica Lam. Caryologia 30: (4) 435–440.
Venkateswarlu, J. & Raja Rao, K. G., 1979. Morphology of the pachytene chromosomes of Physalis pubescens L. Cytologia 44: 161–166.
Wiering, H., De, Vlaming, P., Cornu, A. & Maizonnier, D., 1980. Petunia genetics. I. List of genes. Annls Amél. Pl. 29: 611–622.
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Dietrich, A.J.J., de Jong, J.H. & Mulder, R.J.P. Location and variation of the constitutive heterochromatin in Petunia hybrida. Genetica 55, 85–91 (1981). https://doi.org/10.1007/BF00135102
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DOI: https://doi.org/10.1007/BF00135102