Summary
An immobilization technique has been developed for the conversion of both cellobiose and xylose to ethanol, which may be considered as one stage of a process for the conversion of cellulosic biomass to ethanol. Relatively inexpensive charcoal was used as a support material, with 23 mg dry weight of Clostridium saccharolyticum cells per g dry weight of support. Tests were run for 170 h at 0.15 1/h dilution rate. From a 3% (w/v) sugar mixture, 0.7% (w/v) ethanol was obtained with over 97% cellobiose and 62% xylose utilization.
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Asther, M. and A.W. Khan, 1984a. Biotechnol. Bioeng. 24: 970.
Asther, M. and A.W. Khan, 1984b. Proc. 5th Bioenergy Research and Development Seminar, Ottawa, Canada, March 26–28 (In press).
Asther, M. and A.W. Khan, 1984c. Appl. Microbiol. Biotechnol. (In press).
Chiang, L.C., H.Y. Hsiao, M.C. Flickinger, L.F. Chen and G.T. Tsao, 1982. Enzyme Microb. Technol. 4: 93.
Daugulis, A.J., N.M. Brown, W.R. Cluett and D.B. Dunlop, 1981. Biotechnol. Lett. 3: 651.
Giuliano, C. and A.W. Khan, 1984. Appl. Environ. Microbiol. 48: 446.
Hagerdal, B. and K. Mosbach, 1979. Proc. Food Proc. Eng., Helsinki, Finland, 2: 129.
Horne, P.N. and H.W. Hsu, 1983. Anal. Biochem. 129: 72.
Khan, A.W. and T.M. Trottier, 1978. Appl. Environ. Microbiol. 35: 1027.
Khan, A.W., T.M. Trottier, G.B. Patel and S.M. Martin, 1979. J. Gen. Microbiol. 112: 365.
King, V.A.E. and R.R. Zall, 1983. Proc. Biochem. 18: 17.
Krug, T.A. and A. J. Daugulis, 1983. Biotechnol. Lett. 5: 159.
Luong, J.H.T. and M.C. Tseng, 1984. Appl. Microbiol. Biotechnol. 19: 207.
Margaritis, A. and F.J.A. Merchant, 1984. Critical Rev. in Biotechnol. 1: 339.
Miller, G.L., 1959. Anal. Chem. 31: 426.
Moo-Young, M., J. Lamptey and C.W. Robinson, 1980. Biotechnol. Lett. 2: 541.
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Asther, M., Khan, A.W. Conversion of cellobiose and xylose to ethanol by immobilized growing cells of Clostridium saccharolyticum on charcoal support. Biotechnol Lett 6, 809–812 (1984). https://doi.org/10.1007/BF00134723
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DOI: https://doi.org/10.1007/BF00134723