Summary
Spectrofluorometry was applied to estimate cell number in anchorage dependent cell culture. Ethidium bromide was employed to stain the DNA molecule. Good agreement of cell count was obtained between a Coulter counter and the peak fluorescent intensity of ethidium bromide intercalated within the DNA of lysed cells. This procedure can be utilized to estimate the number of cells growing on surfaces from which trypsinization is ineffective.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey, J.E., J.F. Madjlessi, D.N. McQuitty, L.Y. Lee and J.A. Oro (1978). AIChE J. 24, 570.
Brunner, G., K. Lang, R.A. Wolfe, D.B. McClure and G. Sato (1982). Dev. Brain Res. 2, 563.
Burton, K. (1956). Biochem. J. 62, 315.
Crawford, L.V. and M.J. Waring (1967). J. Mol. Biol. 25, 23.
Ehrlich, K.C., E. Stewart and E. Klein (1978). In Vitro. 14, 443.
Glacken, M.W., R.J. Fleischaker and A.J. Sinskey (1983). Annals New York Academy of Science. 413, 355.
Jensen, M.D. (1981). Biotech. Bioeng. 23, 2703.
Knazek, R.A., P.M. Gullino, P.O. Kohler and R.L. Dedrick (1972). Science. 178, 65.
Ku, K., M.J. Kuo, J. Delente, B.S. Wildi and J. Feder (1981). Biotech.Bioeng. 23, 79.
LePecq, J.B., P. Yot and C. Paoletti (1964). C.R. Acad. Sci. Paris. 259, 1786.
Yguerabide, J. (1978). Spectroscopic and Calorimetric Studies of Viral and Animal Membranes. In: Cell Membranes and Viral Envelopes (eds. Tiffany, J.M. and Blough, H.A.), New York: Academic Press.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tharakan, J.P., Chau, P.C. Cell number estimation via fluorescence measurement. Biotechnol Lett 6, 793–796 (1984). https://doi.org/10.1007/BF00134720
Issue Date:
DOI: https://doi.org/10.1007/BF00134720