Summary
A fed-batch culture of methanol-utilizing microorganism (Corynebacterium sp. XG), a vitamin B 12 producer, was carried out with constant feed of substrate. Experimental results agreed with the theorical model proposed in the literature. Using this feeding system, the final biomass and vitamin B 12 concentration reached 16.3 g/l and 880 μg/l respectively after a 53 h incubation period at 30 °C.
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Andriantsoa, M., Laget, M., Cremieux, A. et al. Constant fed-batch culture of methanol-utilizing corynebacterium producing vitamin B 12. Biotechnol Lett 6, 783–788 (1984). https://doi.org/10.1007/BF00134718
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DOI: https://doi.org/10.1007/BF00134718