Summary
A thermostable inulinase was identified in a strain of A. niger. The highest activity was observed at 50 °C (50 Lml−1) and 77% and 34% of this was retained at 60° and 65°C, respectively. pH stability, the effect of thermal stabilizers such as Propylene glycol (10%) and Sorbitol (10%) and effects of different cations were investigated. It was found that the activity was completely inhibited by Ag+ and Hg2+, while Na+ had an activator effect.
Similar content being viewed by others
References
Azhari, R., Szlak, M.A., Iran, E., Sideman, S. and Lotan, N. (1989). Biotechol. Appl. Biochem., 11, 105–117.
Derycke, G.D. and Vandamme, J.E. (1984). J. Chem. Biotechnol. 34 B, 45–51.
Elyachioui, M., Hornoz J.P. and Tailliez, R. (1992). J. Appl. Bacteriol., 73, 514–519.
Ettalibi, M. and Baratti, J.C. (1990). Agric. Biol. Chem., 54, 61–68.
Fleming, S.E. and Groot Wassink, J.W.D. (1979). CRC Critial Reviews in Food Science and Nutrition. 12(1), 1–28.
Fuchs, A. (1990). InProc. Third Seminar on Inulin (Fuchs, A., ed.), pp. 80–102. NRLO report 90/28, NRLO, The Hague.
Guiraud, J.P., Viard-Gardin, C. and Galzy, P. (1980). Aqric. Biol. Chem., 44, 1245–1252.
Gupta, A.K., Rathore, P., Kaur, N. and Sigh, R. (1990). J. Chem. Tech. Biotechnol., 47, 245–257.
Miller, G.L. (1959). Anal. Chem. 31, 426–428.
Nakamura, T., Kurokawa, T., Nakatsu, S. and Ueda, S. (1978 a). Nippon Nogeikagaku Kaishi 4, 159–166.
Nakamura, T., Maruki, S., Nakatsu, S. and Uedo, S. (1978 b). Nippon Nogeikagaku Kaishi, 52, 12, 581–587.
Norman, E.B. and Zittan, L. (1982). Novo Research Report A-0514.
O'Fagain, C., Sheehan, H., O'Kennedy, R. and Kilty, C. (1988). Proc. Biochem. December 166–171.
Ohta, K., Hamada, S. and Nakamura, T. (1993). Appl. Environ. Microbiol., 59, 3, 729–733.
Parekh, S. and Margaritis, A. (1986). Agric. Biol. Chem., 50, 4, 1985–1087.
Rouwenhorst, P.J., Visser, E.L., Van Der Baan, A.A. and Scheffers, W.A., Van Dijken, P.J. (1988). Appl. Environ. Microbiol., 54, 5, 1131–1137.
Peters, H.J.P.; Kerkhoff, L.P., Calls, H.J.M., Balken, M.A.J. and Mejer, E.M. (1983). DSM Research and Patents Nr. S003723.
Shiomi, N. and Onodera, S. (1988). Agric. Biol. Chem. 52, 10, 2569–2576.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Öngen-Baysal, G., Sukan, Ş.S. & Vassilev, N. Production and properties of inulinase from Aspergillus niger . Biotechnol Lett 16, 275–280 (1994). https://doi.org/10.1007/BF00134625
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00134625