Skip to main content
SpringerLink
Log in

Cloning of the D-lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. cremoris

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

A genomic library from Leuconostoc mesenteroides subsp. cremoris was screened for D-lactate dehydrogenase activity using a stereospecific lactate detection test on agar plate. Among 3500 clones tested, six positive colonies were found on D-lactate detection plate, displaying significantly higher D-LDH activity than Escherichia coli host strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bernard, N., Ferain, T., Gamyn, D., Hols, P., Delcour, J. (1991). FEBS Lett. 290:61–64.

    Google Scholar 

  • Carr, D.B., Shit, V.E., Richter, J.M., Martin, J.B. (1981). Ann. Neurol. 11:195–197.

    Google Scholar 

  • Chen, J.D., Morrison, D.A. (1988). Gene. 64:155–164.

    Google Scholar 

  • Cogan, T.M. (1975). J. Dairy Res. 42:139–146.

    Google Scholar 

  • Cogan, T.M., O'Dowd, M., Mellerick, D. (1981). Appl. Environ. Microbiol. 4:1–8.

    Google Scholar 

  • Collins, E.B. (1972). J. Dairy Sci. 55:1022–1028.

    Google Scholar 

  • De Man, J.C., Rogosa, M., Sharpe, M.E. (1960). Appl. Bacteriol. 23:130–135.

    Google Scholar 

  • Garland, R.C. (1973). Arch. Biochem. Biophys. 157:36–43.

    Google Scholar 

  • Garvie, I.E. (1969). J. Gen. Microbiol. 58:85–94.

    Google Scholar 

  • Jehanno, D., Thuault, D., Bourgeois, J.M. (1992). Appl. Environ. Microbiol. 58:4064–4067.

    Google Scholar 

  • Kochhar, S., Chuard, N., Hottinger, H. (1992). Biochem. Biophys. Res. Comm. 185:705–712.

    Google Scholar 

  • Lin, J., Schmitt, P., Diviès, C. (1991). Appl. Microbiol. Biotechnol. 34:628–631.

    Google Scholar 

  • Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J. (1951). J. Biol. Chem. 193:265–275.

    Google Scholar 

  • Mat-Jam, F., Alam, K.Y., Clark, D.P. (1989). J. Bacteriol. 171:342–348.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F., Manialtis, T. (1989). Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Press.

  • Starenburg, M.J.C., Hugenholtz, J. (1991). Appl. Environ. Microbiol. 57:3535–3540.

    Google Scholar 

  • Tagushi, H., Ohta, T. (1991). J. Biol. Chem. 266:12588–12594.

    Google Scholar 

  • Tarmy, E.M., Kaplan, W.O. (1968). J. Biol. Chem. 243:2579–2586.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Département de Microbiologie-Biotechnologie, ENS.BANA (Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation), Université de Bourgogne, 1 esplanade Erasme, 21000, Dijon, France

    V. Phalip, P. Schmitt & C. Divies

  2. Division of Cellular Biology, The Scripps Clinic Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA

    V. Dartois

Authors
  1. V. Phalip
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Dartois
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Schmitt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Divies
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Phalip, V., Dartois, V., Schmitt, P. et al. Cloning of the D-lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. cremoris . Biotechnol Lett 16, 221–226 (1994). https://doi.org/10.1007/BF00134615

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00134615

Keywords

Search

Navigation