Summary
A flocculant was purified from the culture broth of Archuadendron sp. TS-49 by a series of precipitations with acetone, 60% ammonium sulfate-butanol, salting-out by dialysis, and cetylpyridinium chloride. The flocculating activity was observed most highly at pH 3.0 and markedly enhanced by the addition of salts, especially in the case of FeCl3 or FeSO4. This bioflocculant efficiently flocculated all tested solids, including various microorganisms and organic/ inorganic materials. Qualitative analyses of the bioflocculant showed that it might contain hexosamine, uronic acid, neutral sugar, and protein.
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Lee, S.H., Lee, S.O., Jang, K.L. et al. Microbial flocculant from Arcuadendron sp. TS-49. Biotechnol Lett 17, 95–100 (1995). https://doi.org/10.1007/BF00134203
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DOI: https://doi.org/10.1007/BF00134203