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Selection of fatty acid auxotrophs from the oleaginous yeast Cryptococcus curvatus and production of cocoa butter equivalents in batch culture

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Summary

A search was carried out for mutants, defective in the conversion of stearic acid to oleic acid in effort to improve the quality of lipids produced by Cryptococcus curvatus ATCC, 20509. Mutants were selected as unsaturated fatty acid (Ufa) auxotrophs. After treatment of parent organism with Ethyl methanesulfonate (EMS), 11 oleate-requiring auxotrophs were isolated. Only 3 of them were real unsaturated fatty acid (Ufa) mutants, while the other 8 were designated as fatty acid synthetase (Fas) mutants. The amount of saturated fatty acid (SFA) was about 65.2 % in the lipids extracted from an Ufa mutant named UfaM3 and it was significantly higher than that of the wild-type (WT) (46.6 %) and similar to that of cocoa butter (60.4 %).

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Authors and Affiliations

  1. Département de Génie Biochimique et Alimentaire, URA-CNRS n∘544, Institut National des Sciences Appliquées, Complexe Scientifique de Rangueil, F-31077, Toulouse Cedex, France

    Mainul Hassan, Philippe J. Blanc, Alain Pareilleux & Gérard Goma

  2. Institute of Nutrition and Food Science, University of Dhaka, 1000, Dhaka, Bangladesh

    Mainul Hassan

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  1. Mainul Hassan
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  2. Philippe J. Blanc
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  3. Alain Pareilleux
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  4. Gérard Goma
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Hassan, M., Blanc, P.J., Pareilleux, A. et al. Selection of fatty acid auxotrophs from the oleaginous yeast Cryptococcus curvatus and production of cocoa butter equivalents in batch culture. Biotechnol Lett 16, 819–824 (1994). https://doi.org/10.1007/BF00133960

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