Summary
A triparental conjugation technique (using pRL10630 plasmid) was used in creation and characterization of three transposon-induced mutants of Anabaena PCC7120 which are capable of extracellular ammonia liberation in the absence and /or presence of a glutamate analogue (MSX). Results suggest that such mutants can potentially serve as suitable biofertilizer to support crops without addition of the glutamate analogue.
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Mahasneh, I.A., Mishra, A.K. & Tiwari, D.N. Transposon-induced mutants of the cyanobacterium Anabaena sp. PCC7120 capable of ammonia liberation. Biotechnol Lett 16, 765–770 (1994). https://doi.org/10.1007/BF00133951
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DOI: https://doi.org/10.1007/BF00133951