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A semi-continuous process for the production of human interferon-αc from E. coli using tangential-flow microfiltration and immuno-affinity chromatography

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Summary

A semi-continuous process for the production of human interferon-αc (IFN) from E. coli is described. Harvesting of bacteria, removal of cell debris and concentration were performed by tangentialflow microfiltration (cross-flow microfiltration). Purification was done in a single step by a monoclonal-Ab column. In the process, purified material, 3–9×108 units/mg protein was obtained with a 65% overall yield.

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Authors and Affiliations

  1. InterYeda Ltd., Kiryat Weizmann Park, Ness-Ziona, Israel

    B. Vaks, Y. Mory & J. U. Pederson

  2. Department of Virology, Weizmann Institute of Science, Rehovot, Israel

    O. Horovitz

Authors
  1. B. Vaks
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  2. Y. Mory
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  3. J. U. Pederson
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  4. O. Horovitz
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Vaks, B., Mory, Y., Pederson, J.U. et al. A semi-continuous process for the production of human interferon-αc from E. coli using tangential-flow microfiltration and immuno-affinity chromatography. Biotechnol Lett 6, 621–626 (1984). https://doi.org/10.1007/BF00133826

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  • DOI: https://doi.org/10.1007/BF00133826

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