Fragments of renal carcinoma of the northern leopard frog, Rana pipiens, were cocultured in vitro with small pieces of tadpole heart, frog heart and frog kidney with gyrotory shaking for up to 14 days at 21°C and 28°C. No invasion by renal carcinoma occurred in confrontation cultures at 21°. However, the three normal tissues were invaded by renal carcinoma in confrontation cultures incubated at 28°C. Invasion in vitro by histologically typical renal carcinoma is thus similar to temperature-dependent invasion by the renal carcinoma-derived cell line PNKT-4B and affords an opportunity for the identification of cell or biochemical events which may be activated at invasion-permissive temperature. Cell or biochemical events which are selectively activated and subsequently repressed as the renal tumor is incubated at invasion-permissive and invasion-restrictive temperatures become significant candidates as events involved in, or causal for, malignant invasion.
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References
Billiau, A., Sobis, H., Eyssen, H., and Van den Berghe, H., 1973, Non-infectious intracisternal A-type particles in a sarcoma-positive, leukemia-negative mouse cell line transformed by murine sarcoma virus (MSV). Archiv fur die gesamte Virus forschung, 43, 345–351.
Bracke, M. E., Van Cauwenberge, R. M-L., and Mareel, M. M., 1984, (+)-Catechin inhibits the invasion of malignant fibrosarcoma cells into chick heart in vitro. Clinical and Experimental Metastasis, 2, 161–170.
Di Berardino, M. A., King, T. J., and McKinnell, R. G., 1963, Chromosome studies of a frog renal adenocarcinoma line carried by serial intraocular transplantation. Journal of the National Cancer Institute, 31, 769–789.
Leibovitz, A., 1963, The growth and maintenance of tissue cell cultures in free gas exchange with the atmosphere. American Journal of Hyiene, 78, 173–180
Lucke, B., 1934, A neoplastic disease of the kidney of the frog, Rana pipiens. American Journal of Cancer, 20, 352–379.
Lucke, B., 1934, A neoplastic disease of the kidney of the frog, Rana pipiens. II. On the occurrence of metastasis. American Journal of Cancer, 22, 326–334.
Lucke, B., and Schlunberger, H., 1949, Induction of metastasis of frog carcinoma by increase of environmental temperature. Journal of Experimental Medicine, 89, 269–278.
Mareel, M. M., Bruyneel, E. A., De Bruyne, G. K., Dragonetti, C. H., and Van Cauwenberge, R. M. L., 1982, Growth and invasion: Separate activities of malignant M04 cell populations in vitro. Membranes in Tumour Growth, edited by T. Galeotti, A. Cittadini, G. Neri and S. Papa (Amsterdam: Elsevier Biomedical Press), pp. 223–232.
Mareel, M. M., Bruyneel, E. A., Dragonetti, C. H., De Bruyne, G. K., Van Cauwenberge, R. M-L., Smets, L. A., and Van Rooy, H., 1984, Effect of temperature on invasion of M04 mouse fibrosarcoma cells in organ culture. Clinical and Experimental Metastasis, 2, 107–125.
Mareel, M., Bruyneel, E., Tweedell, K., McKinnell, R., and Tarin, D., 1985, Temperature-dependence of PNKT-4B frog renal carcinoma cell invasion in vitro. Treatment of Metastasis: Problems and Prospects, edited by K. Hellmann and S. A. Eccles (London, Philadelphia: Taylor & Francis), pp. 335–338.
Mareel, M., Kint, J., and Meyvisch, C., 1979, Methods of study of the invasion of malignant C3H-mouse fibroblasts into embryonic chick heart in vitro. Virchows Archiv B: Cell Pathology, 30, 95–111.
McKinnell, R. G., 1973, The Lucke frog kidney tumor and its herpesvirus. American Zoologist, 13, 97–114.
McKinnell, R. G., 1978, Cloning, Nuclear Transplantation in Amphibia (Minneapolis: University of Minnesota Press).
McKinnell, R. G., 1981, The Lucke renal adenocarcinoma: Environmental influences on the tumor with an appendix concerning chemical mutagenesis. Phyletic Approaches to Cancer, edited by C. J. Dawe, J. C. Harshbarger, S. Kondo, T. Sugimura and S. Takayama (Tokyo: Japan Scientific Societies Press), pp. 101–110.
McKinnell, R. G., 1984, The Lucke tumor: A neoplastic disease of frog kidneys with a herpesvirus etiology. Diseases of Amphibians and Reptiles, edited by G. L. Hoff, F. L. Frye and E. R. Jacobson (New York: Plenum Press), pp. 581–605.
McKinnell, R. G., and Cunningham, W. P., 1982, Herpesviruses in metastatic Lucke renal adenocarcinoma. Differentiation, 22, 41–46.
McKinnell, R. G., and Ellis, V. L., 1972, Herpesviruses in tumors of postspawning Rana pipiens. Cancer Research, 32, 1154–1159.
McKinnell, R. G., and McKinnell, B. K., 1968, Seasonal fluctuation of frog renal adenocarcinoma prevalence in natural populations. Cancer Research, 28, 440–444.
McKinnell, R. G., and Tarin, D., 1984, Temperature-dependent metastasis of the Lucke renal carcinoma and its significance for studies on mechanisms of metastasis. Cancer Metastasis Reviews, 3, 373–386.
McKinnell, R. G., Ellis, V. L., Dapkus, D. C., and Steven, L. M., Jr, 1972, Early replication of herpesviruses in naturally occurring frog tumors. Cancer Research, 32, 1729–1732.
McKinnell, R. G., Gorham, E., and Martin, F. B., 1980, Continued diminished prevalence of the Lucke renal adenocarcinoma in Minnesota leopard frogs. American Midland Naturalist, 104, 402–404.
McKinnell, R. G., Gorham, E., Martin, F. B., and Schaad, J. W., 1979, Reduced prevalence of the Lucke renal adenocarcinoma in populations of Rana pipiens in Minnesota. Journal of the National Cancer Institute, 63, 821–824.
Meyvisch, C., and Mareel, M., 1982, Influence of implantation site on M04 cell aggregates on the formation of metastases. Invasion and Metastasis, 2, 51–60.
Naegele, R. F., Granoff, A., and Darlington, R. W., 1974, The presence of the Lucke herpesvirus genome in induced tadpole tumors and its oncogenicity: Koch-Henle postulates fulfilled. Proceedings of the National Academy of Sciences, U.S.A., 71, 830–834.
Rollins, L. A., and McKinnell, R. G., 1980, The influence of glucocorticoids on survival and growth of allografted tumors in the anterior eye chamber of leopard frogs. Developmental and Comparative Immunology, 4, 283–294.
Taylor, A. C., and Kollros, J. J., 1946, Stages in the normal development of Rana pipiens larvae. Anatomical Record, 94, 7–23.
TWeedell, K. S., 1967, Induced oncogenesis in developing frog kidney cells. Cancer Research, 27, 0242–2052.
Tweedell, K. S., 1978, Pronephric tumor cell lines from herpesvirus transformed cells. International Agency for Research on Cancer Scientific Publication, 24, II, 609–616.
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McKinnell, R.G., Bruyneel, E.A., Mareel, M.M. et al. Invasion in vitro by explants of Lucke renal carcinoma cocultured with normal tissue is temperature dependent. Clin Exp Metast 4, 237–243 (1986). https://doi.org/10.1007/BF00133589
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DOI: https://doi.org/10.1007/BF00133589