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Modulation of α-chymotrypsin specificity induced by pyridoxal

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Summary

Chymotrypsin catalyses the hydrolysis of the D-isomers of aromatic amino acids and of glycine methyl esters provided that pyridoxal is present. The corresponding L-isomers still behave as substrates for the enzyme even if pyridoxal decreases the rate of their hydrolysis. This change of enzyme stereospecificity should be taken into account in biotechnological processes.

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Authors and Affiliations

  1. Centre de Recherche INSERM, 60 rue de Navacelles, 34100, Montpellier, France

    A. Previero, F. Kraicsovits, M. Pugnière & M A. Coletti-Previero

Authors
  1. A. Previero
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  2. F. Kraicsovits
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  3. M. Pugnière
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  4. M A. Coletti-Previero
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Previero, A., Kraicsovits, F., Pugnière, M. et al. Modulation of α-chymotrypsin specificity induced by pyridoxal. Biotechnol Lett 3, 571–576 (1981). https://doi.org/10.1007/BF00133436

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  • DOI: https://doi.org/10.1007/BF00133436

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