Skip to main content
Log in

Enzyme polymorphism analyses in three endangered species of Himalayan poppy, Meconopsis (Papaveraceae)

Genetic homogeneity in the endangered Himalayan poppy, Meconopsis

  • Regular Research Papers
  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Populations of Meconopsis paniculata and M. sinuata inhabit open alpine slopes, while those of M. simplicifolia are restricted to alpine meadows of Sikkim Himalaya. These species are threatened and represented by very small populations. Three enzymes namely acid phosphatase, esterase, and glutamate dehydrogenase were tested for six populations each of M. paniculata and M. simplicifolia and single population of M. sinuata to analyse the genetic diversity of these species. In addition, alkaline phosphatases, glucose 6-phosphate dehydrogenase and malate dehydrogenase were tested for M. paniculata populations. The results on the electrophoretic variations revealed that genetic polymorphism is low to absent in all the populations of the three species of Meconopsis investigated, and the species are characterized by genetic homogeneity with fixed alleles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bradford, M.M., 1976. A rapid and sensitive method for quantitation of microgram quantities of proteins utilizing the principle of protein dye-binding. Anal. Biochem. 72: 248–254.

    Google Scholar 

  • Brown, A.H.D., 1978. Isozymes, plant population genetic structure and genetic conservation. Theoret. Appl. Genet. 52: 145–157.

    Google Scholar 

  • Brown, A.H.D., 1979. Enzyme polymorphism in plant populations. Theoret. Population Biol. 15: 1–42.

    Google Scholar 

  • Brown, A.H.D. & G.F. Moran, 1981. Isozymes and genetic resources of forest trees. In: M.T. Conkle (Ed.), Isozymes of North American Forest Trees and Forest Insects, pp. 1–10. U.S. Department of Agriculture, Berkeley.

    Google Scholar 

  • Brown, A.H.D., E. Nevo, D. Zohary & O. Dagan, 1978. Genetic variation in natural populations of wild barley (Hordeum spontaneum). Genetica 49: 97–108.

    Google Scholar 

  • Cooper, T.G. 1977. The Tools of Biochemistry, pp. 227, Wiley, New York.

    Google Scholar 

  • Crisp, M.D. 1988. Eucalyptus recurva (Myrtaceae), a new species from the Southern Tablelands of New South Wales. Telopea 3: 223–230.

    Google Scholar 

  • Davis, B.J., 1964. Disc electrophoresis' II. Methods and application to human serum proteins. Annu. Rev. N. Y. Acad. Sci. 121: 404–427.

    Google Scholar 

  • Debnath, H.S. & M.P. Nayar, 1986. The Poppies of Indian Region (Papaveraceae). Botanical Survey of India, Calcutta.

    Google Scholar 

  • Fowler, D.P. & R.W. Morris, 1977. Genetic diversity in red pine. Evidence for low heterozygosity. Can. J. Forest Res. 7: 341–347.

    Google Scholar 

  • Hartmann, T., M. Nagel & H.I. Ilert, 1973. Organ specific multiple forms of glutamic dehydrogenase in Medicago sativa. Planta 111: 119–128.

    Google Scholar 

  • Karron, J.D., Y.B. Linhart, C.A. Chaulk & C.A. Robertson, 1988. Genetic structure of populations of geographically restricted and widespread species of Astragalus (Gabaceae). Amer. J. Botany 75: 1114–1119.

    Google Scholar 

  • Ledig, F.T. & M.T. Conkle, 1983. Gene diversity and genetic structure in a narrow endemic, Torrey pine (Pinus torreyana Parry ex Car). Evolution 37: 79–85.

    Google Scholar 

  • Lundkvist, K. & D. Rudin, 1977. Genetic variation in eleven population of Picea abies as determined by isozyme analysis. Hereditas 85: 67–74.

    Google Scholar 

  • McClenaghan, L.R. & A.C. Beauchamp, 1986. Low genetic differentiation among isolated populations of the Californian fan Palm (Washingtonia filifera). Evolution 40: 315–322.

    Google Scholar 

  • Moran, G.F. & S.D. Hopper, 1987. Conservation of the genetic resources of rare and widespread eucalyptus in remnant vegetation. In D.A. Saunders, G.W. Arnold, A.A. Burbidge & A.J.M. Hopkins (Eds.), pp. 151–162, Nature Conservation: The Role of Remnant Vegetation, Surrey Beatty and Sons, NSW.

    Google Scholar 

  • Moran, G.F., Muona, O. & J.C. Bell, 1989. Acacia mangium: a tropical forest tree of the coastal lowlands with low genetic diversity. Evolution 43: 231–235.

    Google Scholar 

  • Nei, M., 1972. Genetic distance between populations. Amer. Naturalist 106: 283–292.

    Google Scholar 

  • Poverene, M.M. & N.R. Curvetto, 1989. Esterase isozyme variation in the Eragrostis curvula complex (Poaceae). Plant Systematics and Evolution 166: 173–181.

    Google Scholar 

  • Prober, S.M., C. Tompkins, G.F. Moran & J.C. Bell, 1990. The conservation genetics of Eucalyptus paliformis L. Johnson et Blaxell and E. parvifolia Cambage, Two rare species from Southeastern Australia. Austral. J. Botany 38: 79–95.

    Google Scholar 

  • Sampson, J.F., S.D. Hopper & S.H. James, 1988. Genetic diversity and the conservation of Eucalyptus crucis Maiden. Austral. J. Botany 36: 447–460.

    Google Scholar 

  • Scandalios, J.G., 1969. Genetic control of multiple molecular forms of enzymes in plants. A review. Biochem. Genet. 3: 37–79.

    Google Scholar 

  • Scandalios, J.G., 1979. Control of Gene Expression and Enzyme Differentiation, pp. 63–107. Physiological Genetics. Academic Press, New York.

    Google Scholar 

  • Schwaegerle, K.E. & B.A. Schaal, 1979. Genetic variability and founder effect in the pitcher plant Sarracenia purpurea. Evolution 36: 361–370.

    Google Scholar 

  • Sing, C.F. & G.J. Brewer, 1969. Isozymes of polyploid series of wheat. Genetics 61: 391–398.

    Google Scholar 

  • Sulaiman, I.M., 1992. Conservation Biological Studies on Meconopsis Vig. Species Populations. Ph.D. Thesis, University of Delhi, Delhi, India.

    Google Scholar 

  • Sulaiman, I.M., 1993. Seed germination studies in three species of threatened, ornamental, Himalayan poppy, Meconopsis Vig. (Papaveraceae). Seed Science and Technology 21: 593–603.

    Google Scholar 

  • Sulaiman, I.M., 1994. Regeneration of plantlets through organogenesis in the Himalayan yellow poppy, Meconopsis paniculata. Plant Cell Tissue and Organ Culture 36: 377–380.

    Google Scholar 

  • Sulaiman, I.M. & C.R. Babu, 1993. In vitro regeneration through organogenesis of Meconopsis simplicifolia — An endangered ornamental species. Plant Cell Tissue and Organ Culture 34: 295–298.

    Google Scholar 

  • Sulaiman, I.M., N.S. Rangaswamy & C.R. Babu, 1991. Formation of plantlets through somatic embryogeny in the Himalayan blue poppy, Meconopsis simplicifolia (Papaveraceae). Plant Cell Reports 9: 582–585.

    Google Scholar 

  • Tostain, S. & L. Marchais, 1989. Enzyme diversity in pearl millet (Pennisetum glaucum), 2. Africa and India. Theoret. Appl. Genet. 77: 634–640.

    Google Scholar 

  • Waller, D.M., D.M. O'Malley & S.C. Gawler, 1987. Genetic variation in the extreme endemic Pedicularis furbishiae (Scrophulariaceae). Conservation Biol. 1: 335–340.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sulaiman, I.M., Babu, C.R. Enzyme polymorphism analyses in three endangered species of Himalayan poppy, Meconopsis (Papaveraceae). Genet Resour Crop Evol 43, 351–356 (1996). https://doi.org/10.1007/BF00132955

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00132955

Key words

Navigation