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Utilization of free and immobilized Desulfovibrio hydrogenase in hydrogen photoproduction : Coupling efficiency of cytochrome C3, ferredoxin and flavodoxin

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Summary

Hydrogen photoproduction has been achieved by coupling free or immobilized hydrogenases from Desulfovibrio species to illuminated chloroplasts through different electron mediators. Whereas D. gigas flavodoxin or ferredoxin I cannot directly mediate the electron flux from chloroplasts to hydrogenase, the addition of these mediators considerably enhances the H2 photoproduction of a system including cytochrome C3. These immobilized hydrogenases exhibit good stability under working conditions and can be re-used.

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Author notes

  1. M. F. Cocquempot

    Present address: Laboratoire de Biotechnologie Solaire, CEN de Cadarache, BP n∘ 1, 13114, Saint Paul Les Durance, France

Authors and Affiliations

  1. Laboratoire de Chimie Bactérienne, CNRS, 2, Marseille, France

    R. Aguirre & E. C. Hatchikian

  2. Département de Génie Biochimique et Alimentaire, ERA CNRS n∘ 879 INSA, Avenue de Rangueil, 31077, Toulouse, France

    P. Monsan

  3. Laboratoire de Technologie Enzymatique, ERA CNRS n∘ 338, U.T.C. BP, 233 - 60206, Compiègne, France

    M. F. Cocquempot & T. Lissolo

Authors
  1. R. Aguirre
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  2. E. C. Hatchikian
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  3. P. Monsan
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  4. M. F. Cocquempot
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  5. T. Lissolo
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Aguirre, R., Hatchikian, E.C., Monsan, P. et al. Utilization of free and immobilized Desulfovibrio hydrogenase in hydrogen photoproduction : Coupling efficiency of cytochrome C3, ferredoxin and flavodoxin. Biotechnol Lett 4, 297–302 (1982). https://doi.org/10.1007/BF00132829

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  • DOI: https://doi.org/10.1007/BF00132829

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