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Production of 6-aminopenicillanic acid by immobilized Pleurotus ostreatus

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Summary

6-Aminopenicillanic acid from penicillin V is produced by Pleurotus ostreatus immobilized by entrapment in a chitosan matrix. In these carriers the cell concentration increases after network formation by irreversible shrinking of the biocatalyst. Specific activity of the biocatalyst for the hydrolysis reaction is 1,31 μmol.min-1. (g wet weight of catalyst)-1 corresponding to a relative activity of 38%. Catalytic half-life of immobilized Pl. ostreatus is 25 days compared to 2.5 days for free suspended cells.

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Authors and Affiliations

  1. Institute of Chemical Technology, Technical University Braunschweig, Hans-Sommer-Strasse 10, D 3300, Braunschweig, Fed. Rep. of Germany

    Michael Kluge & Joachim Klein

  2. Technical University Braunschweig, Konstantin-Uhde-Str. 8, D 3300, Braunschweig, Fed. Rep. Germany

    Fritz Wagner (Chair of Biochemistry and Biotechnology)

Authors
  1. Michael Kluge
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  2. Joachim Klein
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  3. Fritz Wagner
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Additional information

This paper is part of the Dissertation of Michael Kluge, Technical University Braunschweig 1981.

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Kluge, M., Klein, J. & Wagner, F. Production of 6-aminopenicillanic acid by immobilized Pleurotus ostreatus. Biotechnol Lett 4, 293–296 (1982). https://doi.org/10.1007/BF00132828

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  • DOI: https://doi.org/10.1007/BF00132828

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