Abstract
The Drosophila retrotransposon, 412, is expressed in a cell-specifie manner during embryogenesis. At stage 11, 412 transcripts are present in bilateral clusters of cells within the mesoderm. The posterior clusters of 412-positive cells become associated with the gonads at stage 13; however, the fate of the cells in the remaining clusters is unknown. We have tested byin situ hybridization to whole-mount embryos the possible identity of these cells with known precursor cell types present in bilateral clusters. We simultaneously located the 412-positive cells and the precursor cells to visceral muscle or the fat body. We have determined that the 412-positive cells do not correspond to these precursor cells and that the development of the visceral muscle or fat body does not affect the expression of 412 during embryogenesis.
References
Abel, T., A. M. Michelson & T. Maniatis, 1993. A Drosophila GATA family member that binds to Adh regulatory sequences in expressed in the developing fat body. Dev. 119:623–633.
Azpiazu, N. & M. Frasch, 1993. Tin and bagpipe: two homeobox genes that determine cell fates in the dorsal mesoderm ofDrosophila. Genes Dev. 7:1325–1340.
Brookman, J. J., A. T. Toosy, L. S. Shashidhara & R. White, 1992. The 412 retrotransposon and the development of gonadal mesoderm inDrosophila. Dev. 116: 1185–1192.
Ding, D. & H. D. Lipshitz, 1994. Spatially regulated expression of retrovirus-like transposons duringDrosophila melanogster embryogenesis. Genet. Res. Camb. 64: 167–181.
Hoshizaki, D. K., T. Blackburn, C. Price, M. Ghosh, K. Miles, M. Ragucci & R. Sweis, 1994. Embryonic fat-cell lineage inDrosophila melanogaster. Dev. 120: 2489–2499.
Hoshizaki, D. K. & D. J. Finnegan, 1985. Behavior of aDrosophila melanogaster transposable element inSaccharomyces cerevisae. Mol. Cell. Biol. 5:3325–3329.
Hoshizaki, D. K., R. Lunz, M. Ghosh & W. Johnson, 1995. Identification of fat-cell enhancer activity inDrosophila melanogaster using P-element enhancer traps. Genome (in press).
Hoshizaki, D. K., 1994. Kruppel expression during postembryonic development ofDrosophila. Dev. Biol. 163:133–140.
Jiang, J., D. Kosman, Y. T. Ip & M. Levine, 1991. The morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos. Genes Dev. 5:1881–1891.
Lindsley, D. & G. Zimm (eds), 1992. The genome ofDrosophila melanogaster. Academic Press, New York.
Lloyd, A. & S. Sakonju, 1991. Characterization of two Drosophila POU domain genes, related to oct-1 and oct-2, and the regulation of their expression patterns. Mech. Dev. 36:87–102.
Parkhurst, S. & V. Corces, 1987. Developmental expression ofDrosophila melanogaster retrovirus-like transposable elements. EMBO J. 6:419–424.
Tautz, D. & C. Pfeifle, 1989. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals a translational control of the segmentation gene hunchback. Chromosoma 98:81–85.
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Tan, C., Price, C. & Hoshizaki, D.K. 412-Positive mesodermal cells and the gonadal mesoderm are separate from the fat-cell lineage. Genetica 97, 111–115 (1996). https://doi.org/10.1007/BF00132587
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DOI: https://doi.org/10.1007/BF00132587