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The influence of internal and external diffusional limitations on the observed kinetics of immobilized whole bacterial cells with cell-associated β-glucosidase activity

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Summary

Kinetic studies of β-glucosidase activity of immobilized Alcaligenes faecalis indicated the presence of internal diffusional limitations by an increase in Vmax and η, and a decrease in Km′ as particle size decreased. A small effect of external diffusional limitation in a batch reactor was noted as Vmax decreased and Km′ increased with stirrer speed.

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Authors and Affiliations

  1. Department of Chemical Engineering and Applied Chemistry, University of Toronto, M5S 1A4, Toronto, Ontario, Canada

    Margaret A. Wheatley & Colin R. Phillips

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  1. Margaret A. Wheatley
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  2. Colin R. Phillips
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Wheatley, M.A., Phillips, C.R. The influence of internal and external diffusional limitations on the observed kinetics of immobilized whole bacterial cells with cell-associated β-glucosidase activity. Biotechnol Lett 5, 79–84 (1983). https://doi.org/10.1007/BF00132163

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  • DOI: https://doi.org/10.1007/BF00132163

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