Summary
A kanamycin-resistant plasmid possessing a thermostable replicon derived from Bacillus stearothermophilus cryptic plasmid pSTK1 was constructed. The plasmid could transform not only B. stearothermophilus and Bacillus subtilis, but also Gram-negative Escherichia coli. The behavior of the plasmid in the hosts was examined. The plasmid was stably maintained even at 67°C in B. stearothermophilus without selective pressure. During the plasmid replication, single-stranded DNA (ssDNA) intermediates were found in E. coli, while these were not found in B. subtilis.
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Narumi, I., Nakayama, N., Nakamoto, S. et al. Bacillus stearothermophilus plasmid pSTK1 replicon is functional in Escherichia coli . Biotechnol Lett 17, 475–480 (1995). https://doi.org/10.1007/BF00132013
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DOI: https://doi.org/10.1007/BF00132013