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Covalent coupling of glutamate dehydrogenase of Aspergillus ochraceus on zirconia-coated alkylamine glass

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Summary

The immobilized glutamate dehydrogenase of Aspergillus ochraceus on alkylamine glass exhibited 39% of the initial enzyme activity and a conjugation yield of 13.6 mg enzyme protein/g support. The Km of the bound enzyme varied while Vmax remained unaltered. The immobilized enzyme was optimally active at pH 8.6 with greater potential for prolonged and repeated use.

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Authors and Affiliations

  1. Division of Biochemistry, Central Drug Research Institute, 226001, Lucknow, India

    A. K. Agrawal, Gyan P. Srivastava, Chanan Singh & V. K. Mohan Rao

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  1. A. K. Agrawal
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  2. Gyan P. Srivastava
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  3. Chanan Singh
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  4. V. K. Mohan Rao
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Agrawal, A.K., Srivastava, G.P., Singh, C. et al. Covalent coupling of glutamate dehydrogenase of Aspergillus ochraceus on zirconia-coated alkylamine glass. Biotechnol Lett 5, 615–618 (1983). https://doi.org/10.1007/BF00130843

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