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Induction of β-N-acetylhexosaminidase in Aspergillus oryzae

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Summary

Extracellular β-N-acetylhexosaminidase in basic specific activity 1.5 U/mg protein was induced 15 – 35 times (up to 50 U/mg protein) by mixture of chitooligomers (crude chitin hydrolysate), 10 – 20 times (20 – 30 U/mg protein) by N-acetylglucosamine, and 10 times (14 U/mg protein) by chitosan in Aspergillus oryzae. Addition of NaCl (15 – 23 g/l) to the cultivation medium enhanced the induction in 10 – 20 %.

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Authors and Affiliations

  1. Institute of Organic Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, D-20146, Hamburg, Germany

    O. Scheel & J. Thiem

  2. Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídeňská 1083, CZ 142 20, 4, Prague, Czech Republic

    Z. Huňková, V. Křen, M. Ščigelová, L. Weignerová, O. Scheel & J. Thiem

Authors
  1. Z. Huňková
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  2. V. Křen
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  3. M. Ščigelová
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  4. L. Weignerová
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  5. O. Scheel
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  6. J. Thiem
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Huňková, Z., Křen, V., Ščigelová, M. et al. Induction of β-N-acetylhexosaminidase in Aspergillus oryzae . Biotechnol Lett 18, 725–730 (1996). https://doi.org/10.1007/BF00130773

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  • DOI: https://doi.org/10.1007/BF00130773

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