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Ethanol production by recombinant Escherichia coli KO11 using crude yeast autolysate as a nutrient supplement

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Summary

Crude yeast autolysate (10 g/l; 5.8 g solubles/l) supplemented with minerals and vitamins served as a nutrient supplement for ethanol production by the recombinant bacterium, Escherichia coli KO11. Ethanol production in this medium (46±1.7 g ethanol/l) was equivalent to that obtained using 5–15 g/l of purified, soluble, commercial protein hydrolysates (44–48 g ethanol/l). On site production of autolysates from spent yeast offers the potential for synergy between grain-based and lignocellulose-based ethanol plants.

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Authors and Affiliations

  1. Institute of Food and Agricultural Science Department of Microbiology & Cell Science, University of Florida, Box 110700, 32611, Gainesville, Florida, USA

    S. W York & L. O. Ingram

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  1. S. W York
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  2. L. O. Ingram
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York, S.W., Ingram, L.O. Ethanol production by recombinant Escherichia coli KO11 using crude yeast autolysate as a nutrient supplement. Biotechnol Lett 18, 683–688 (1996). https://doi.org/10.1007/BF00130766

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  • DOI: https://doi.org/10.1007/BF00130766

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