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Optimization of PCR template concentrations for in vitro transcription-translation reactions

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Summary

In vitro transcription-translation reactions can be used to rapidly and inexpensively synthesize small amounts of protein from cloned DNA sequences for biochemical studies. The use of PCR to amplify template sequences has many advantageous over conventional protocols. However, the amount of template needs to be carefully adjusted for optimal production of protein products.

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References

  • Guiltinan, M.J., Marcotte, W.R., Jr., and Quatrano, R.S. (1990). Science 250, 267–271.

    Google Scholar 

  • Guiltinan, M.J., and Miller, L. (1994). Plant Molecular Biology 26, 1041–1053.

    Google Scholar 

  • Nishikawa, B., Fowlkes, D., and Kay, B. (1989). Biotechniques 7, 730–734.

    Google Scholar 

  • Schagger, H., and Jagow, G. von (1987). Analytical Biochemistry 166, 368–379.

    Google Scholar 

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Authors and Affiliations

  1. Department of Horticulture and The Biotechnology Institute, 306 Wartik Lab, 16802, University Park, PA, USA

    Lisa Renshaw-Gegg & Mark J. Guiltinan

  2. The Pennsylvania State University, 306 Wartik Lab, 16802, University Park, PA, USA

    Mark J. Guiltinan

Authors
  1. Lisa Renshaw-Gegg
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  2. Mark J. Guiltinan
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Renshaw-Gegg, L., Guiltinan, M.J. Optimization of PCR template concentrations for in vitro transcription-translation reactions. Biotechnol Lett 18, 679–682 (1996). https://doi.org/10.1007/BF00130765

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  • DOI: https://doi.org/10.1007/BF00130765

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