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Effect of nitrogen sources on the regulation of extracellular lipase production in Acinetobacter calcoaceticus strains

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Summary

Extracellular lipase production and lipA expression were studied in A.calcoaceticus strains in relation to the nitrogen sources. Addition of casamino-acids or tryptone improved 2–3 times lipase yield as compared to ammonium, yeast extract or proteose-peptone. Further increase in lipase yield and enzyme stability were achieved when ammonium was also added. Differences in yields were not related to lipA transcription. Proteolytic activity was detected in culture broths simultaneous with lipase degradation. Post- transcriptional processes including enzyme protection, inactivation and secretion must be considered as important factors affecting lipase production.

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Authors and Affiliations

  1. Catedra de Biotecnología. Facultad de Farmacia y Bioquímica. UBA., Junín 956, 1113, Buenos Aires, Argentina

    Alfredo Cordenons, Rodrigo Gonzalez & Clara Nudel

  2. Dept. Microbiology, EC Slater Institute. University of Amsterdam, Nieuwe Achtergracht 127, 1018, Amsterdam, WS, The Netherlands

    Ruben Kok & Klaas J. Hellingwerf

Authors
  1. Alfredo Cordenons
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  2. Rodrigo Gonzalez
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  3. Ruben Kok
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  4. Klaas J. Hellingwerf
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  5. Clara Nudel
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Cordenons, A., Gonzalez, R., Kok, R. et al. Effect of nitrogen sources on the regulation of extracellular lipase production in Acinetobacter calcoaceticus strains. Biotechnol Lett 18, 633–638 (1996). https://doi.org/10.1007/BF00130756

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  • DOI: https://doi.org/10.1007/BF00130756

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