Summary
To evaluate the biological effects of gas pollutants on alveolar macrophages several in vitro systems ave been developed. We described here an original method of cell culture in aerobiosis, which permitted direct contact between the atmosphere and the target cells. We studied the long term (24 h) and short term (30 min) effects of NO2 on alveolar macrophages. Our results demonstrated that exposure of alveolar macrophages to gas pollutants may be responsible for either cell injury or cell activation associated with the release of various bioactive mediators (superoxide anion, neutrophil chemotactic activity). Cell culture in aerobiosis opens new ways for the research on the biological effects of gas pollutants.
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Abbreviations
- AM:
-
alveolar macrophages
- CL:
-
Chemiluminescence
References
ALINK, G.M., VAN DER HOEVEN, J.C.M., DEBETS, F.M.H., VAN DE VEN, W.S.M., and KOEMAN, J.H. (1979). A new exposure model for in vitro testing of effects of gaseous pollutants on mammalian cells by mean of gas diffusion through plastic films. Chemosphere 2:63–73.
BOLTON, D.C., TARKINGTON, B.K., ZEE, Y.C., and OSEBOLD, J.W. (1982). An in vitro system for studying the effects of ozone on mammalian cell cultures and viruses. Environ Res. 27:466–475.
BRODY, A.R. (1986). Pulmonary cell interactions with asbestos fibers in vivo and in vitro. Chest. 89: 1555–1595.
DENICOLA, D.B., REBAR, A.H., and HENDERSON, R.F. (1981). Early damage Indicators in the lung. V. Biochemical and cytological response to NO2 inhalation. Toxicol Appl Pharmacol 60:301–312.
EHRLICH, R. (1966). Effect of nitrogen dioxide on resistance to respiratory infection. Bact Rev. 30:604–614.
FELS, A.O.S. and COHN, Z.A. (1986). The alveolar macrophage. J Appl Physiol. 60:353–369.
GUERBERO, R.R., ROUNDS, D.E., BOOHER, J., OLSON, R.S., and MACKNEY, T.D. (1979a). Ozone sensitivity in against WII-38 cells based on acid phosphatase content. Arch Environm Health 34:407–412.
GUERBORO, R.R., ROUNDS, D.E., OLSON, R.S., and MAKNEY, J.D. (1979b). Mutagenic effects of ozone on human cells exposed in vivo and in vitro based in sister chromatid exchange analysis. Environ Res. 18: 336–346.
HENRY, M.C., EHRLICH, R., and BLAIR, W.H. (1969). Effect of nitrogen dioxide on resistance of squirrel monkeys to Klebsiella pneumonia infection. Arch Environm Health 18:580–587.
MOHSENIN, V. and GEE, B.L. (1987). Acute effect of nitrogen dioxide exposure on the functional activity of Alpha-1-protease inhibitor in bronchoalveolar lavage fluid or normal subjects. Am Rev Respir Dis. 136:646–650.
MURLAS, C.G. and ROUM, J.H. (1985). Sequence of pathologic changes in the airway mucosa of guinea pigs during ozone-induced bronchial hyperaeactivity. Am Rev Respir Dis. 131:314–320.
O'BYRNE, P.M., WALTERS, E.H., GOLD, B.D., AIZAWA, H.A., FABBRI, L.M., ALPERT, S.E., NADEL, J.A. and HOLTZMAN, M.J. (1984). Neutrophil depletion inhibits airway hyper responsiveness induced by ozone exposure. Am Rev Respir Dis. 130:214–219.
OREHEK, J., MASSARI, J.P., GAYRARD, P., GRIMAUD, C., and CHARPIN, J. (1976). Effects of short-term, low levels nitrogen dioxide exposure on bronchial sensitivity of asthmatic patients. J Clin Invest 57: 301–307.
PARAZZI, E., SECCHI, G.C., PERNIS, B., and VIGLIANI, E. (1968). Studies on the cytotoxic action of silica dusts on macrophages “in vitro”. Arch Environ Health 17:850–855.
PATEL, J.M. and BLOCK, E.R. (1986). Nitrogen Dioxide-induced changes in cell membrane fluidity and function. Am Rev Respir Dis. 134:1196–1202.
RASMUSSEN, R.E. In vitro system for exposure of lung cells to NO2 and O3. (1984). J Toxicol Environm Health 13:397–411.
RICHTER, A.M., ABBOUD, R.T., JOHAL, S.S., and FERA, T.A. (1986). Acute effect of smoking on superoxide production by pulmonary alveolar macrophages. Lung 1164:233–242.
SCHEULE, R.K. and HOLIAN, A. (1999). Immunologic aspects of pneumoconiosis. Exp Lung Res. 17: 661–685.
SONE, S., BRENNAN, L.M., and CREASIA, D.A. (1983). In Vitro and in vitro NO2 exposures enhance phagocytic and tumoricidal activities of rat alveolar macrophages. J Toxicol Environm Health 11:151–161.
VOISIN, C., AERTS, C., and HOUDRET, J.L. (1974). Méthode d'étude des effets du NO2 sur les macrophages alvéolaires de cobaye en survie in vitro. Rev Fr Mal Resp 2 (suppl. 1):93–97.
VOISIN, C., AERTS, C., JAKUBSCZAK, E., and TONNEL, A.B. (1977). La culture cellulaire en phase gazeuse. Un nouveau modéle expérimental d'étude in vitro des activités des macrophages. Bull Europ Physiopathol Respir 13:69–82.
VOISIN, C., AERTS, C., JAKUBSZAK, E., HOUDRET, J.L., and TONNEL, A.B. (1977). Effects du bioxyde d'azote sur les macrophages alvéolaires en phase gazeuse. Un nouveau modèle expérimental pour l'étude in vitro de la cytotoxicité des gaz nocifs. Bull Eur Physiopathol Respir 13: 137–144.
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Wallaert, B., Voisin, C. In vitro study of gas effects on alveolar macrophages. Cell Biol Toxicol 8, 151–156 (1992). https://doi.org/10.1007/BF00130522
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DOI: https://doi.org/10.1007/BF00130522