The Sertoli cell extends from the basement membrane of the seminiferous tubule towards its lumen; it sends cytoplasmic processes which envelop different generations of germ cells. The use of Sertoli cell culture began to develop in 1975. To reduce germ cell contamination immature animals are generally used as Sertoli cell donors. Sertoli cell mitosis essentially occurs in sexually immature testes in mammals; mitosis of these cells is observed in vitro during a limited period of time. Sertoli cells in vivo perform an impressive range of functions: structural support of the seminiferous epithelium, displacement of germ cells and release of sperm; formation of the Sertoli cell blood-testis barrier; secretion of factors and nutrition of germ cells; phagocytosis of degenerating germ cells and of germ cell materials. Some of the Sertoli cell functions can be studied in vitro. The recent development of Sertoli cell culture on permeable supports (with or without extracellular matrix) has resulted in progress in understanding the vectorial secretion of several Sertoli cell markers. In addition to FSH and testosterone, several other humoral factors are known to influence Sertoli cell function. Furthermore, myoid cells bordering the tubules as well as germ cells are capable of regulating Sertoli cell activity. Sertoli cells are the most widely used testicular cells for in vitro toxicology. The testis is highly vulnerable to xenobiotics and radiations, yet the number of studies undertaken in this field is insufficient and should be drastically increased.
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AILENBERG, M., TUNG, P.S., PELLETIER, M., and FRITZ, I.B. (1988). Modification of Sertoli cell function in two-chamber assembly by peritubular cells and extracellular matrix.Endocrinology. 122:2604–2612.
BARDIN, C.W., CHENG, C.Y., MUSTO, N.A., and GUNSALUS, G.L. (1988). “The Sertoli cell.” In: The Physiology of Reproduction. (E. Knobil, J. Neill et al., eds.). Vol 1, pp. 933–974. Raven Press, New York.
BYERS, S.W., HADLEY, M.A., DJAKIEW, D., and DYM, M. (1986). Growth and characterization of polarized monolayers of epididymal epithelial cells and Sertoli cells in dual environment culture chambers. J. Androl. 7:59–68.
CHAPIN, R.E. and PHELPS, J. (1990). Recent advances in testicular cell culture: implications for toxicology. Toxic. in Vitro. 4:543–559.
CLERMONT, Y. and PEREY, B. (1957). Quantitative study of the cell population of the seminiferous tubules in immature rats. Am. J. Anat. 100:241–266.
DE KRETSER, D.M. and KERR, J.B. (1988). The Cytology of the Testis. In: The Physiology of Reproduction. (E. Knobil, J. Neill et al., eds.). Vol 1, pp. 837–932. Raven Press, New York.
DORRINGTON, J.H. and ARMSTRONG, D.T. (1975). Follicle-stimulating hormone stimulates oestradiol-17β synthesis in cultured Sertoli cells. Proc. Natl. Acad. Sci. USA. 72:2677–2681.
FAWCETT, D.W. (1975). Ultrastructure and function of the Sertoli cell. In: Handbook of Physiology. Endocrinology. (D.W. Hamilton, R.D. Greep, eds.). Section 7. Vol. V, pp. 21–55. Williams and Wilkins Co, Baltimore.
GRISWOLD, M.D., SOLARI, A., TUNG, P.S., and FRITZ, I.B. (1977). Stimulation by follicle-stimulating hormone of DNA synthesis of mitosis in cultured Sertoli cells prepared from testis of immature rats. Mol. Cell. Endocr. 7:151–165.
HADLEY, M.A., DJAKIEW, D., BYERS, S.W., and DYM, M. (1987). Polarized secretion of androgen-binding protein and transferrin by Sertoli cells grown in a bicameral culture system. Endocrinology. 120:1097–1103.
JANECKI, A. and STEINBERGER, A. (1986). Polarized Sertoli cell functions in a new two-compartment culture system. J. Androl. 7:69–71.
JANECKI, A., JAKUBOWIAK, A., and STEINBERGER, A. (1988). Effect of germ cells on vectorial secretion of androgen-binding protein and transferrin by immature rat Sertoli cells in vitro. J. Androl. 9:126–132.
JÉGOU, B. (1991). Spermatids are regulators of Sertoli cell function. In: The Male Germ Cell: Spermatogonium to Fertilization. (B. Robaire, ed.). Ann. NY Acad. Sci., Vol 637, pp. 340–353. Acad. Sci., New York.
JéGOU, B. (1992). The Sertoli Cell Function. In: The Testis. (D.M. de Kretser, ed.). Vol. 6, pp. 273–311. Baillière's Clinical Endocrinology and Metabolism.
JÉGOU, B., LE MAGUERESSE, B., SOURDAINE, P., PINEAU, C., VELEZ DE LA CALLE, J.F., GARNIER, D.H., GUILLOU, F., and BOISSEAU, C. (1987). Germ cell-Sertoli cell interactions in vertebrates. In: Molecular and Cellular Endocrinology of the Testis. (B. Cooke, R.M. Sharpe, eds.). Sereno Symposia Publications. V 30, pp. 255–270. Raven Press, New York.
JÉGOU, B., SYED, V., SOURDAINE, P., BYERS, S., GÉRARD, N., VELEZ DE LA CALLE, J.F., PINEAU, C., GARNIER, D.H., and BAUCHÉ, F. (1992). The dialogue between late spermatids and Sertoli cells in vertebrates: a century of research. In: Spermatogenesis-Fertilization-Contraception. Molecular cellular and Endocrine Events in Male Reproduction. (E. Nieshlag et al., eds.). pp. 57–95. Shering Foundation Series, Springer.
KODANI, M. and KODANI, K. (1965). The in vitro cultivation of mammalian Sertoli cells. Proc. Natl. Acad. Sci. USA. 56:1200–1206.
KOSKIMIES, A.I., and KORMANO, M. (1973). The proteins in the fluids from the seminiferous tubules in rete testis of the rat. J. Reprod. Fertil. 34:433–444.
LE MAGUERESSE, B., and JÉGOU, B. (1988). In vitro effects of germ cells on secretory activity of Sertoli cells recovered from rat of different ages. Endocrinology. 122:1672–1680.
PINEAU, C., LE MAGUERESSE, B., COUTENS, J.L., and JÉGOU, B. (1991). Study in vitro of the phagocytic function of Sertoli cells in the rat. Cell Tissue Res. 264:589–598.
Ploën, L. and Setchell, B.P. (1992). Blood-testis barriers revisited. A homage to Lennart Nicander. Int. J. Androl. 15:1–4.
SERTOLI, E. (1865). Dell' esistenza di particulari cellule ramificate nei canalicoli seminiferi dell' testiculo umano. Morgagni. 7:31–39.
SKINNER, M.K. (1991). Cell-cell interactions in the testis. Endocrine Rev. 12:45–77.
STEINBERGER, A. and STEINBERGER, E. (1971). Replication pattern of Sertoli cells in maturing rat testis in vivo and in organ culture. Biol. Reprod. 4:84–87.
STEINBERGER, A., HEINDEL, J.J., LINDSEY, J.N., ELKINGTON, J.S.H., SANBORN, B., and STEINBERGER, E. (1975). Isolation and culture of FSH-responsive Sertoli cells. Endocrine Res. Commun. 2: 261–272.
VOGL, A.W., PFEIFFER, D.C., and REDENBACH, D.M. (1991). Ectoplasmic (“junctional”) specializations in mammalian Sertoli cells: influence on spermatogenic cells. In: The Male Germ Cell Spermatogonium to Fertilization. (B. Robaire, ed). V. 637, pp. 175–202. Ann. N.Y. Acad. Sciences.
WIEBE, J.P., BUCKINGHAM, K.D., WOOD, P.H., and CAMPBELL, S.M.C. (1987). Relative steroidogenic activity of Sertoli and Leydig cells and role of the Sertoli cell steroid 3α-hydroxy-4-pregnen-20-one, in spermatogenesis and FSH secretion. In: Development and Function of the Reproductive Organs. (M. Parvinen, I. Hutaniemi, and L.J. Pelliniemi, ed.). Vol. II, pp. 39–53. Serono Symposia; Review N° 14. Ares Serono Symposia, Rome.
ZHUANG, L.Z., PHILLIPS, D.M., GUNSALUS, G.L., BARDIN, C.W., and MATHER, J.P. (1983). Effect of Gossypol on Rat Sertoli and Leydig cells in primary culture and established cell lines. J. Androl. 4: 336–344.
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Jégou, B. The Sertoli cell in vivo and in vitro . Cell Biol Toxicol 8, 49–54 (1992). https://doi.org/10.1007/BF00130510
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DOI: https://doi.org/10.1007/BF00130510