Summary
A local isolate of Bacillus thuringiensis (B.t.81) produced crystal protein which was identified as a cry I gene of Class I. The synthesis and assembly of crystal complements were investigated at intervals throughout the growth cycle. Incubation temperature had a marked effect on toxin synthesis; production being the highest at 25°C and the lowest at 42°C. The mutants of B. t. 81, unable to synthesize crystal protein complements, have also been described.
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Afkhami, P., Özcengiz, G. & Alaeddinoglu, N.G. Production of the lepidoptera-specific crystal protein by a local isolate of Bacillus thuringiensis . Biotechnol Lett 15, 1253–1258 (1993). https://doi.org/10.1007/BF00130307
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DOI: https://doi.org/10.1007/BF00130307