Summary
The size distribution of cell aggregates, and the effect of cell aggregate size on anthocyanin content of Daucus carota cells in suspension cultures, was studied. The profile of biomass distribution in various size groups of cell aggregates indicated that over 92% of biomass was present in the aggregates of 500–1500 μm in diameter. The anthocyanin content increased initially with the increase in cell aggregate diameter up to 500–850 μm, and decreased rapidly with the increase in the cell aggregate size above this critical diameter. On the other hand, the surface colour intensity showed a steady increase with the increase in cell aggregate size, indicating a steep radial gradient of anthocyanin content along the radius of the larger cell aggregates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bowler,C. and Chua,N.M. (1994). Plant Cell 6, 1529–1541.
Buitelaar,R.M. and Tramper,J. (1992). J. Biotechnol. 23, 111–141.
Dixon,R.A. (1985). Isolation and maintenance of callus and cell suspension cultures. In: Plant Cell Culture. R.A.Dixon, ed. pp 1–20, Oxford: IRL Press.
Doran,P.M. (1993). Adv. Biochem. Eng. Biotechnol. 48, 115–168.
Gana,J.A., Sharma,G.C., Zipf,A., Saha,S., Roberts,J. and Wesenburg,D.M. (1995). Plant Cell Tissue Organ Cult. 40, 217–224.
Hulst,A.C., Meyer,M.M.T., Breteler,H. and Tramper,J. (1989). Appl. Microb. Biotechnol. 30, 18–25.
King,P.J., Cox,B.J., Fowler,M.W. and Street,H.E. (1974). Planta 117, 109–122.
Lindsey,K. and Yeoman,M.M. (1983). J. Exp. Bot. 34, 1055–1065.
Mavituna,F. and Park,J.M. (1987). Chem. Eng. J. 35, 9–14.
Murashige,T. and Skoog,F. (1962). Physiol. Plant. 15, 473–497.
Nassau,K. (1993). Color. In: Encyclopaedia of Chemical Technology, M.Howe-Grant, ed., vol. 6. pp 841–876, New York: John Wiley & Sons, Inc.
Panda,A.K., Mishra,S., Bisaria,V.S. and Bhojwani,S.S. (1989). Enz. Microb. Technol. 11, 386–397.
Rajendran,L., Suvarnalatha,G., Ravishankar,G.A. and Venkataraman,L.V. (1994). Appl. Microb. Biotechnol. 42, 227–231.
Spier,R.E. (1995). Enz. Microb. Technol. 17, 91–92.
Stafford,A. (1994). Plant Sci. 101, 91–98.
Steward,F.C., Mapes,M.O. and Mears,K. (1958). Amer. J. Bot. 45, 705–708.
Stickland,G.G. and Sunderland,N. (1972). Ann. Bot. 36, 443–457.
Street,H.E. (1977). Plant Tissue and Cell Culture, Oxford: Blackwell.
Takayama,S., Misawa,M., Ko,K. and Misato,T. (1977). Physiol. Plant. 41, 313–320.
Tanaka,H., Ayogi,H. and Jitsufuchi,T. (1992). J. Ferment. Bioeng. 73, 130–134.
Tanaka,H., Semba,H., Jitsufuchi,T. and Harada,H. (1988). Biotechnol. Let. 10, 485–490.
Zhong,J.J., Seki,T., Kinoshita,S. and Yoshida,T. (1991). Biotechnol. Bioeng. 38, 653–658.
Zhong,J.J., Yoshida,M., Fujiyama,K., Seki,T. and Yoshida,T. (1993). J. Ferment. Bioeng. 75, 299–303.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Madhusudhan, R., Ravishankar, G.A. Gradient of anthocyanin in cell aggregates of Daucus carota in suspension cultures. Biotechnol Lett 18, 1253–1256 (1996). https://doi.org/10.1007/BF00129949
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00129949