Skip to main content
SpringerLink
Log in

Effect of Trichoplusia ni BTI-Tn 5B1-4 cell density on human secreted alkaline phosphatase production

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

The effect of Tn 5B1-4 cell density at infection on the production of human secreted alkaline phosphatase (SEAP) was investigated using a split-flow air-lift bioreactor. Volumetric productivities of SEAP were highest when Tn 5B1-4 cells were infected at about 3 × 105 cells/cm2. Cell-to-cell contact inhibition is an important factor responsible for the reduced protein production at high cell densities. Other factors such as oxygen or nutrient limitation, or build up of metabolic inhibitors do not appear to be as important.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wickham, T.J., Davis, T., Granados, R.R., Shuler, M.L. and Wood, H.A. (1992) Biotechnol. Prog. 8, 391–396

    Google Scholar 

  2. Davis, T.R., Wickham, T.J., McKenna, K.A., Granados, R.R., Shuler, M.L. and Wood, H.A. (1992) In Vitro Cellular and Developmental Biology. In Press.

  3. Wickham, T.J. and Nemerow, G.R. (1993) Biotechnol. Prog, 9, 25–30.

    Google Scholar 

  4. Chung, I.S., Taticek, R., and Shuler, M.L. (1993) Biotechnol. Prog. In Press.

  5. Lazarte, J.E., Tosi, P.F. and Nicolau, C. (1992) Biotechnol. Bioeng. 40, 214–217.

    Google Scholar 

  6. Vaughn, J.L. (1976) J. Invertebr. Pathol. 26, 233–237.

    Google Scholar 

  7. Volkman, L.E. and Summers, M.D. (1975) J. Virol. 16, 1630–637.

    Google Scholar 

  8. Hink, W.F., Strauss, E.M. and Ramoska, W.A. (1977) J. Invertebr. Pathol. 30, 185–191.

    Google Scholar 

  9. Stockdale, H. and Gardiner, G.R. (1977) J. Invertebr. Pathol. 30, 330–336.

    Google Scholar 

  10. Wood, H.A., Johnston, L.B. and Burand, J.P. (1982) Virol. 119, 245–254

    Google Scholar 

  11. Lindsay, D.A. and Betenbaugh, M.J. (1992) Biotechnol. Bioeng. 39, 614–618

    Google Scholar 

  12. Caron, A.W., Archambault, J. and Massie, B. (1990) Biotechnol. 36, 1133–1140.

    Google Scholar 

  13. Davis, T.R., Trotter, K.M., Granados, R.R. and Wood, H.A. (1992) Bio/technology 10, 1148–1150.

    Google Scholar 

  14. Wood, H.A. (1977) J. Invertebr. Pathol. 29, 304–307.

    Google Scholar 

  15. Berger, J., Hauber, J., Geigewr, R. and Cullen, B.R. (1988) Gene 66, 1–10.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Chemical Engineering, Cornell University, 14853, Ithaca, NY, USA

    I. S. Chung & M. L. Shuler

  2. Department of Genetic Engineering, Kyung Hee University, Suwon, Korea

    I. S. Chung

Authors
  1. I. S. Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. L. Shuler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chung, I.S., Shuler, M.L. Effect of Trichoplusia ni BTI-Tn 5B1-4 cell density on human secreted alkaline phosphatase production. Biotechnol Lett 15, 1007–1012 (1993). https://doi.org/10.1007/BF00129927

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00129927

Keywords

Search

Navigation