Production of an indolizidine alkaloid, swainsonine by the filamentous fungus, Metarhizium anisopliae

Summary

The physiology of Metarhizium anisopliae has been studied with a view to improving swainsonine production. In preliminary work, the stability of pure swainsonine in aqueous solution has been investigated under varying conditions of pH and temperature and in the presence of oxidizing agents. The effects of oxygen on the growth and morphology of M.anisopliae and the production of swainsonine were also examined. Shaken flask fermentations have been carried out in order to develop a medium suitable for production of swainsonine.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Donaldson, M.J., Broby,H., Adlard, M.W. & Bucke, C. (1990). Phytochemical Analysis 1: 18–21.

    Google Scholar 

  2. 2.

    Dorling, P.R., Huxtable, C.R. and Colegate, S.M. (1980). Biochem. J. 191: 649–651.

    Google Scholar 

  3. 3.

    Elbein, A.D. & Molyneux, R.J. (1987). Alkaloids 5: 1–54.

    Google Scholar 

  4. 4.

    Fellows, L.E. & Nash, R.J. (1990). Sci. Progress 2(294): 245–255.

    Google Scholar 

  5. 5.

    Guengerich, F.P. & Broquist, H.P. (1973). Biochemistry 12(21): 4270–4274.

    Google Scholar 

  6. 6.

    Guengerich, F.P., Dimari, S.J. and Broquist, H.P. (1973). J.Am. Chem. Soc. 95(6): 2055–2056.

    Google Scholar 

  7. 7.

    Harris, C.M., Schneider, M.J., Ungemach, F.S., Hill, J.E. and Harris, T.M. (1988). J.Am. Chem. Soc. 110: 940–949.

    Google Scholar 

  8. 8.

    Hino, M., Nakayama, O., Tsurumi, Y., Adachi, K., Shibata, T., Terano, H., Kohsaka, M., Aoki, H. & Imanaka, H. (1985). J.Antibiot. 38: 926–935.

    Google Scholar 

  9. 9.

    Tulsiani, D.R.P., Broquist, H.P. & Touster, O. (1985). Archives of Biochem. & Biophys. 236(1): 427–434.

    Google Scholar 

  10. 10.

    Wickwire, B.M., Wagner, C. & Broquist, H.P. (1990). J.Biol. Chem. 265(25): 14748–14753.

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Patrick, M., Adlard, M.W. & Keshavarz, T. Production of an indolizidine alkaloid, swainsonine by the filamentous fungus, Metarhizium anisopliae . Biotechnol Lett 15, 997–1000 (1993). https://doi.org/10.1007/BF00129924

Download citation

Keywords

  • Oxygen
  • Aqueous Solution
  • Fermentation
  • Organic Chemistry
  • Alkaloid