Advertisement

Biotechnology Letters

, Volume 15, Issue 10, pp 997–1000 | Cite as

Production of an indolizidine alkaloid, swainsonine by the filamentous fungus, Metarhizium anisopliae

  • M. Patrick
  • M. W. Adlard
  • T. Keshavarz
Article

Summary

The physiology of Metarhizium anisopliae has been studied with a view to improving swainsonine production. In preliminary work, the stability of pure swainsonine in aqueous solution has been investigated under varying conditions of pH and temperature and in the presence of oxidizing agents. The effects of oxygen on the growth and morphology of M.anisopliae and the production of swainsonine were also examined. Shaken flask fermentations have been carried out in order to develop a medium suitable for production of swainsonine.

Keywords

Oxygen Aqueous Solution Fermentation Organic Chemistry Alkaloid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Donaldson, M.J., Broby,H., Adlard, M.W. & Bucke, C. (1990). Phytochemical Analysis 1: 18–21.Google Scholar
  2. 2.
    Dorling, P.R., Huxtable, C.R. and Colegate, S.M. (1980). Biochem. J. 191: 649–651.Google Scholar
  3. 3.
    Elbein, A.D. & Molyneux, R.J. (1987). Alkaloids 5: 1–54.Google Scholar
  4. 4.
    Fellows, L.E. & Nash, R.J. (1990). Sci. Progress 2(294): 245–255.Google Scholar
  5. 5.
    Guengerich, F.P. & Broquist, H.P. (1973). Biochemistry 12(21): 4270–4274.Google Scholar
  6. 6.
    Guengerich, F.P., Dimari, S.J. and Broquist, H.P. (1973). J.Am. Chem. Soc. 95(6): 2055–2056.Google Scholar
  7. 7.
    Harris, C.M., Schneider, M.J., Ungemach, F.S., Hill, J.E. and Harris, T.M. (1988). J.Am. Chem. Soc. 110: 940–949.Google Scholar
  8. 8.
    Hino, M., Nakayama, O., Tsurumi, Y., Adachi, K., Shibata, T., Terano, H., Kohsaka, M., Aoki, H. & Imanaka, H. (1985). J.Antibiot. 38: 926–935.Google Scholar
  9. 9.
    Tulsiani, D.R.P., Broquist, H.P. & Touster, O. (1985). Archives of Biochem. & Biophys. 236(1): 427–434.Google Scholar
  10. 10.
    Wickwire, B.M., Wagner, C. & Broquist, H.P. (1990). J.Biol. Chem. 265(25): 14748–14753.Google Scholar

Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • M. Patrick
    • 1
  • M. W. Adlard
    • 1
  • T. Keshavarz
    • 1
  1. 1.School of Biological and Health Sciences, Faculty of Engineering and ScienceUniversity of WestminsterLondonUK

Personalised recommendations