Skip to main content
Log in

Mapping of two plasmids from Lactobacillus helveticus ILC 54, plasmid curing and preliminary studies on their involvement in lactose metabolism and peptidase activity

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Two plasmids isolated from Lactobacillus helveticus strain ILC 54 were analysed by restriction digestion. A restriction map of the 14.3 and 5.6 kb plasmids is presented. Plasmid-curing studies suggest that these plasmids are involved in lactose metabolism and peptidase activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderson, D.G. and McKay, L.L. (1983). Appl. Environm. Microbiol. 46, 549–552.

    Google Scholar 

  • Bradford, M.M. (1976). Anal. Biochem. 72,248–254.

    Google Scholar 

  • Church, F.C., Swaisgood, H.E., Portier, D.H. and Catignani, G.L. (1983). J. Dairy Sci. 66, 1219–1227.

    Google Scholar 

  • De Man, J.C., Rogosa, M. and Sharpe, M.E. (1960). J. Appl. Bacteriol. 23, 130–135.

    Google Scholar 

  • De Rossi, E., Brigidi, P., Riccardi, G., Milano, A. and Matteuzzi, D. (1989). Microbiologica 12, 273–276.

    Google Scholar 

  • El Soda, M. and Desmazeaud, M.J. (1982). Can. J. Microbiol. 28, 1181–1188.

    Google Scholar 

  • Exterkate, F.A. (1975). Neth. Milk Dairy J. 29, 303–318.

    Google Scholar 

  • Fortina, M.G., Parini, C., Manachini, P.L., Morelli, L., Bottazzi, V. and Concari, P. (1990). Biotechnol. Lett. 12, 765–770.

    Google Scholar 

  • Fortina, M.G., Parini, C., Rossi, P. and Manachini, P.L. (1993). Lett. Appl. Microbiol. 17, 303–306.

    Google Scholar 

  • Hintermann, G., Fischer, H.M., Crameri, R. and Hutter, R. (1981). Plasmid 5, 371–373.

    Google Scholar 

  • Maniatis, T., Fritsch, E.F. and Sambrrok, J. (1982). Molecular cloning: a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.

    Google Scholar 

  • Morelli, L., Vescovo, M., Cocconcelli, P.S. and Bottazzi, V. (1986). Can. J. Microbiol. 32, 758–760.

    Google Scholar 

  • Smiley, M.B. and Fryder, D. (1978). Appl. Environm. Microbiol. 35, 777–781.

    Google Scholar 

  • Takiguchi, R., Hashiba, H., Aoyama, K. and Ishii, S. (1989). Appl. Environm. Microbiol., 55, 1653–1655.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fortina, M.G., Silva, M. Mapping of two plasmids from Lactobacillus helveticus ILC 54, plasmid curing and preliminary studies on their involvement in lactose metabolism and peptidase activity. Biotechnol Lett 18, 1003–1006 (1996). https://doi.org/10.1007/BF00129721

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00129721

Keywords