Abstract
A strain of Bacillus amyloliquefaciens was cultivated in a membrane recycle bioreactor for the production of an extracellular α-amylase. Continuous cultivations of B. amyloliquefaciens in the recycle fermentor led to higher cell mass and volumetric productivities than the ones obtained in batch or chemostat cultivations; the α-amylase activities were lower than in the batch mode but significantly higher than in the chemostat mode. The operation of the membrane recycle bioreactor was sometimes disturbed by high broth viscosity leading to a stronger fouling of the membrane.
Similar content being viewed by others
References
Bruner, R.L.(1964). Meth. Carbohydr. Chem. 4, 67–71.
Denis, S. and Boyaval, P. (1991). Appl. Microbiol. Biotechnol., 34, 608–612
Hammarberg, B., Nagamune, T., Endo, I., Moks, T. and Uhlén, M. (1991). Bioproc. Eng. 7, 145–150
Kawakatsu, T., Miwa, T., Matsumoto, Y., Nakao, S.I. and Kimura, S. (1993). J. Ferment. Bioeng. 75, 126–131.
Kroner, K.H., Schütte, H., Hustedt, H. and Kula, M.R. (1984). Proc. Biochem. 4, 67–74.
Nipkow, A., Zeikus, J.G. and Gerhardt, P. (1989). Biotechnol. Bioeng. 34, 1075–1084.
Pirt, S.J. and Kurowski, W.M.(1970). J. Gen. Microbiol. 63, 357–366.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Morcel, C., Emborg, C., Madsen, S. et al. α-Amylase production by Bacillus amyloliquefaciens in a membrane recycle bioreactor. Biotechnol Lett 17, 643–648 (1995). https://doi.org/10.1007/BF00129393
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00129393