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Effect of water-miscible aprotic solvents on kyotorphin synthesis catalyzed by immobilized α-chymotrypsin

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Summary

Immobilized α-chymotrypsin was used as catalyst to synthesize a kyotorphin derivative (Bz-Tyr-Arg-OEt) in the presence of five water-miscible aprotic solvents (dimethylsulphoxide, dimethylformamide, acetonitrile, acetone and tetrahydrofurane) at 30 °C. By using a kinetically-controlled approach, the maximum synthetic activity was obtained when Arg-OEt was used as nucleophile donor at a concentration 1.5-times higher than the acyl-acceptor substrate (Bz-Tyr-OEt). The water-miscible aprotic solvents enhanced greatly the synthetic activity proportionally to their hidrophilicity properties adequately measured by the log P parameter. At the optimum solvent concentration for the enzymatic peptide synthesis, both the water activity (Aw) of the media and the water content of the immobilized derivative showed a saturation profile against the log P parameter. As a function of the solvent hydrophilicity, these water parameters were shown as key parameters for the increase in the synthetic activity of the enzyme by the presence of these solvents.

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References

  • Batra, R. and Gupta, M.N. (1994). Biotechnol. Lett., 16, 1059 - 1064.

    Google Scholar 

  • Blanco, R.M., Rakels, J.L, Guisán, J.M. and Halling, P. (1992). Biochim. Biophys. Acta., 1156, 67 - 70.

    Google Scholar 

  • Cerovsky, V. and Jakubke, H.D. (1994). Enzyme Microb. Technol., 16, 596 - 601.

    Google Scholar 

  • Flörsheimer, A., Kula, M.R., Shütz, H.J. and Wandrey, C. (1989). Biotechnol. Bioeng., 33, 1400 - 1405.

    Google Scholar 

  • Kise, H., Fujimoto, K. and Noritomi H. (1988). J. Biotechnol., 8, 279 - 290.

    Google Scholar 

  • Lozano, P., Iborra, J.L., Manjón, A. and Combes, D. (1992). Biotechnol. Lett., 14, 933 - 936.

    Google Scholar 

  • Lozano, P., Cano, J., Iborra, J.L. and Manjón, A. (1993). Biotechnol. Appl. Biochem., 18, 67 - 74.

    Google Scholar 

  • Lozano, P., Combes, D. and Iborra, J.L. (1994). J. Biotechnol., 35, 9 - 18.

    Google Scholar 

  • Mozhaev, V.V., Khmelnitsky, Y.L., Sergeeva, M.V., Belova, A.B., Klyachko, N.L., Levashov, A.V. and Martinek, K. (1989). Eur. J. Biochem., 184, 597 - 602.

    Google Scholar 

  • Nagashima, T., Watanabe, A. and Kise, H. (1992). Enzyme Microb. Technol., 14, 842 - 847.

    Google Scholar 

  • Reslow, M., Adlercreutz, P. and Mattiason, B. (1988). Eur. J. Biochem., 172, 573 - 588.

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular B e Inmunología. Facultad de Química, Universidad de Murcia, P.O. Box. 4021, E-30001, Murcia, Spain

    Pedro Lozano, Teresa Diego & José L. Iborra

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  1. Pedro Lozano
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  2. Teresa Diego
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  3. José L. Iborra
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Lozano, P., Diego, T. & Iborra, J.L. Effect of water-miscible aprotic solvents on kyotorphin synthesis catalyzed by immobilized α-chymotrypsin. Biotechnol Lett 17, 603–608 (1995). https://doi.org/10.1007/BF00129386

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  • DOI: https://doi.org/10.1007/BF00129386

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