Skip to main content
SpringerLink
Log in

Fungal protoplasts as tools for studies on protein secretion

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Protoplasts were prepared from mycelium of Aspergillus niger N-402. Sucrose was used to induce the synthesis and secretion of invertase. Protoplasts secreted 2 forms of invertase, different to those secreted by the mycelium. 14C mannose was shown to be taken up by protoplasts and incorporated into secreted proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Archer, B.D.(1994).In Recombinant Microbes for Industrial and Agricultural Applications; (.eds Murooka, Y., Imanaka, T.) pp. 373–393. New York. Marcel Dekker.

    Google Scholar 

  • Bennett, J.W.(1985).In:Fungal Protoplasts - Applications in Biochemistry and Genetics, (.eds Peberdy, J.F. & Ferenczy, L.) pp. 189–203. New York, Marcel Dekker.

    Google Scholar 

  • Berges,T.,Barreau,C.,Peberdy,J.F.,Boddy,L.M. (1993)Current Genetics. 24, 53–59.

    Google Scholar 

  • Boddy,L.M.,Berges,T.,Barreau,C.,Vainstein,M.T.,Dobson,M.J.,Ballance,D.J., Peberdy,J.F.(1993). Curr. Genet. 24, 60–68.

    Google Scholar 

  • Buzzi, M., Felipe,M.S.S., Azevedo, M.O., Caldas,R.A. (1993). J. Gen. Microbiol. 139, 1885–1889.

    Google Scholar 

  • Chen, J.S., Saxton, J.W.,Hemming, F.W., Peberdy, J.F. (1996) Biochem.Biophys.Acta. (in press).

  • Davis, R.W. (1991). In:Applied Molecular Genetics of Fungi, (.eds. Peberdy, J.F.,Caten,C.E.,Ogden, J.E.,Bennett,J.W.) pp. 103–117. Cambridge. Cambridge University Press.

    Google Scholar 

  • Fawcett, P.A.,Loder, P.B., Duncan, M.J.,Beesley,T.J.,Abraham,E.P. (1973). J. Gen.Microbiol. 79, 293–309.

    Google Scholar 

  • Elorza,M.V.,Marcilla,A.,Sanjuan,R.,Mormeneo,S.,Sentandreu,R.,Stelles,G. (1994).Arch.Microbiol. 161, 145–151.

    Google Scholar 

  • Gabriel, O. & Wang,S.F. (1969).Anal. Biochem. 27, 545–555.

    Google Scholar 

  • Hamlyn,P.F.,Bradshaw,R.E.,Mellon,F.M.,Santiago,C.M.,Wilson.J.M., Peberdy,J.F. (1981). Enzyme Microb. Technol. 3, 321–325.

    Google Scholar 

  • Hemming,F.W. (1995).Biochemical Society Transactions. 23, 180–185.

    Google Scholar 

  • Isaac,S. (1985). In:Fungal Protoplasts - Applications in Biochemistry and Genetics, (eds Peberdy, J.F. & Ferenczy,L.)6, pp. 171–187. New York, Marcel Dekker.

    Google Scholar 

  • Laemmli, U.K. (1970). Nature. 227, 680–683.

    Google Scholar 

  • Lowry,O.H.,Rosebrough,N.J.,Farr,A.L.,Randall,R.J.(1951).193, 265–275.

  • Matsuoka,H.,Teraoka,T.,Sumita,O.,Takatori,O.,Kurata,H. (1993). Mycopathol. 122, 135–141.

    Google Scholar 

  • Newbury, J.A. & Peberdy, J.F. (1996) Mycological Research (in press).

  • Oxenbøll, K. (1993) In: The Genus Aspergillus: from Taxonomy and Genetics to Industrial Application.(eds Powell, K.A.,Renwick, A., & Peberdy, J.F.) pp 147–154. New York,Plenum Press).

    Google Scholar 

  • Peberdy, J.F. (1993). J. Chem Technol Biotechnol. 56, 216–217.

    Google Scholar 

  • Peberdy, J.F. (1994).Tibtech. 12, 50–57.

    Google Scholar 

  • Pontecorvo, G.,Roper,J.A.,Hemmons, L.M.,MacDonald, K.D.,Bufton, A.W.J. (1953). Adv. in Genet. 5, 141–238.

    Google Scholar 

  • Towbin,H.,Staehelin,T.,Gordon,J. (1979).Proc. Nat. Acad.Sci. USA. 76, 4350–4354.

    Google Scholar 

  • Ushijima,S.,Nakadai,T.,Uchida,K. (1991).Agric. Biol. Chem. 55, 129–136.

    Google Scholar 

  • Vaguolgyi,C. & Ferenczy,L. (1991)FEMSMicrobiol. Lett. 82, 247–252.

    Google Scholar 

  • Vainstein, M.H. & Peberdy, J.F. (1990)FEMSMicrobiol. Lett. 71, 265–270

    Google Scholar 

  • Vainstein,M.H. & Peberdy,J.F. (1991a)J. Gen.Microbiol. 137, 315–321.

    Google Scholar 

  • Vainstein, M.H. & Peberdy, J.F. (1991b)Mycol. Res. 95, 1270–1274

    Google Scholar 

  • Wösten,H.A.B.,Moukha,S.M.,Sietsma,J.H.,Wessels,G.H. (1991).J. Gen.Microbiol. 137, 2017–2023.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK

    T. K. Sørensen, G. L. F. Wallis & J. F. Peberdy

Authors
  1. T. K. Sørensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. L. F. Wallis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. F. Peberdy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sørensen, T.K., Wallis, G.L.F. & Peberdy, J.F. Fungal protoplasts as tools for studies on protein secretion. Biotechnol Lett 18, 1375–1380 (1996). https://doi.org/10.1007/BF00129338

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00129338

Keywords

Search

Navigation