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Intergeneric protoplast fusion between Acinetobacter sp. A3 and Pseudomonas putida DP99 for enchanced hydrocarbon degradation

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Summary

Polyethylene glycol 6000 mediated protoplast fusion between an alkane degrader Acinetobacter sp. A3, and a naphthalene degrader, Pseudomonas putida DP99 , resulted in fusants capable of degrading both hydrocarbons and were morphologically similar to Acinetobacter sp. A3. While fusant F4/13 and Pseudomonas putida DP99 degraded over 98% of naphthalene provided by the end of five days, tetradecane degradation by fusant F4/13 was 82% compared to 77% by Acinetobacter sp. A3 in the same time period. Also, while from naphthalene +tetradecane mixture, fusant F4/13 could degrade 99% and 53% of naphthalene and tetradecane respectively, both the parent strains together could degrade over 99% naphthalene but only about 16% tetradecane.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology Centre, M.S. University of Baroda, 390 002, Baroda, India

    Hanson K. G. & Anjana J. Desai

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  1. Hanson K. G.
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  2. Anjana J. Desai
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Hanson, K.G., Desai, A.J. Intergeneric protoplast fusion between Acinetobacter sp. A3 and Pseudomonas putida DP99 for enchanced hydrocarbon degradation. Biotechnol Lett 18, 1369–1374 (1996). https://doi.org/10.1007/BF00129337

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  • DOI: https://doi.org/10.1007/BF00129337

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